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生草对花椒园土壤养分及微生物丰度的影响

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2024-12-23 16:44

Li P H, Zeng P, Gong X, et al. Effects of sod-culture on soil nutrients and microbial abundance in Zanthoxylum bungeanum plantation[J]. Journal of Sichuan Forestry Science and Technology, 2021, 42(4): 73−77. DOI: 10.12172/202012100002

Citation: Li P H, Zeng P, Gong X, et al. Effects of sod-culture on soil nutrients and microbial abundance in Zanthoxylum bungeanum plantation[J]. Journal of Sichuan Forestry Science and Technology, 2021, 42(4): 73−77. DOI: 10.12172/202012100002

生草对花椒园土壤养分及微生物丰度的影响

四川省植物工程研究院，四川资中 641200

详细信息

作者简介:

李佩洪（1983—），女，副研究员，硕士研究生，lipeihong_58@163.com

通信作者:

陈政: 19941953@163.com

计量文章访问数: 0396 HTML全文浏览量: 0122 PDF下载量: 021 出版历程收稿日期: 2020-12-09 网络出版日期: 2021-05-26 刊出日期: 2021-08-24

Effects of Sod-culture on Soil Nutrients and Microbial Abundance in Zanthoxylum bungeanum Plantation

Sichuan Academy of Botanical Engineering, Zizhong 641200, China

More Information

Corresponding author:

CHEN Zheng: 19941953@163.com

摘要

摘要: 在花椒林下种植白三叶、培育自然杂草，以清耕为对照，研究了花椒林下土壤养分、土壤微生物的变化。结果表明：生草对花椒园土壤养分及微生物丰度的影响主要在0~20 cm土层；生草提高了花椒林土壤有机质含量，增加了部分营养元素的含量，增加了土壤中微生物的丰度，改变了微生物的种群结构；生草初期可能对土壤水分和氮肥的消耗较大，应根据花椒树的生长特性结合农艺措施进行科学的管理。

关键词: 生草 / 土壤养分 / 土壤微生物

Abstract: In order to study the impacts of sod-culture on soil nutrients and soil microorganisms under Zanthoxylum bungeanum plantation, white clover was planted and natural weeds were cultivated, and the changes of soil nutrients and soil microorganisms were studied with clear tillage as control. The results showed that the effect of grass on soil nutrients and microbial abundance was mainly in 0~20 cm soil layer in Zanthoxylum bungeanum plantation. Grass increased the content of soil organic matter, some nutrient elements, the microbial abundance in soil, and changed the population structure of microorganisms. At the early stage of grass growing, the consumption of soil water and nitrogen fertilizer may be great, so scientific management combined with agricultural measures should be carried out according to the growth characteristics of Zanthoxylum bungeanum.

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图 1 各水平微生物分类单元数统计图

Figure 1. Statistical diagram of the number of microbial classification unit at each level

图 2 属水平物种的相对丰度图

Figure 2. Relative abundance diagram of species composition at genus level

表 1 土壤养分测定结果

Table 1 Result of soil nutrients

覆盖方式土层深度
cm水分含量
%有机质
%总氮
%总磷
%全钾
%铵态氮
mg·kg−1有效磷
mg·kg−1有效钾
mg·kg−1钙
g·kg−1有效硼
mg·kg−1 白三叶草 0—2010.359.440.0530.1052.1027.672.7325.873.400.3120—4013.772.850.0360.1132.4546.152.5424.321.800.2840—6016.131.960.0250.1182.1249.034.0624.862.700.32平均值13.424.750.0380.1122.2240.953.1125.022.630.30自然生草 0—2011.966.650.0530.1742.5546.703.3033.142.400.3020—4011.995.30.0330.1242.2654.402.8725.002.600.3340—6015.625.160.0300.1491.8753.062.3326.41.100.25平均值13.195.700.0390.1492.2351.392.8328.182.030.29对照 0—2017.033.510.0630.1292.2267.313.1931.363.700.4220—4016.931.970.0350.1051.8858.783.4720.951.500.2340—6016.462.050.0290.1122.0145.994.3720.192.800.15平均值16.812.510.0420.1152.0457.363.6824.172.670.27

表 2 每样本测序量统计表

Table 2 Statistical result of sequencing quantity per sample (tag)

SampleInputFilteredDenoisedMergedNon-chimericNon-singleton clover1172069157392156970156243150679150679Weeds1170017155299154737153513145790145789CK1141849129199128973128453127447127447Clover2124910114358114150113144112226112226Weeds2139312127879127647126960122652122652CK2147702135143134961134633134391134388Clover3158470144592144444143839143483143483Weeds3162882146566146435145564145398145398CK3163033146125146020145241145227145227 　　注：Sample为样本名，Input为原始数据量，Filtered为去除低质量序列后的数据量，Denoised为有效序列量；Merged为拼接后的序列量，Non-chimeric为高质量序列量，Non-singleton为去除singleton后的序列量；1、2和3分别代表0~20 cm、20~40 cm和40~60 cm的土层深度。
　　Note: Sample is the sample name, Input is the original data quantity, Filtered is the data quantity after removing the low-quality sequence, and Denoised is the effective sequence quantity; Merged is the sequence quantity after splicing, Non-chimeric is the high quality sequence quantity, and Non-singleton is the sequence quantity after singleton is removed. 1, 2 and 3 represent the soil depth of 0~20 cm, 20~40 cm and 40~60 cm, respectively.

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