ABT处理对‘美人’梅插穗几种内含物含量的影响
摘要 将‘美人’梅(Prunus × blireana ‘Meiren’)嫩枝插穗进行200 mg/L ABT生根剂处理，以清水处理作为对照，对插穗在生根过程中的外部形态、营养物质含量、抗氧化酶活性和内源激素含量的变化情况进行对比和分析.结果显示：(1)插穗从开始扦插到形成大量不定根的整体所需时间约为28 d；(2)ABT对插穗的生根具有显著的有利影响；(3)ABT处理的插穗，其可溶性糖、可溶性蛋白、脯氨酸(Proline, Pro)、生长素(Indole-3-acetic Acid, IAA)和赤霉素(Gibberellins, GA)含量在扦插后期后均显著高于CK；(4)对于超氧化物歧化酶(Superoxide Dismutase, SOD)活性的变化趋势，处理和CK之间无明显的差异.通过分析可认为：‘美人’梅插穗生根需满足高含量的IAA和低含量的GA，前期较高的可溶性糖和可溶性蛋白含量可促进不定根的诱导和发育.ABT处理的插穗在扦插中后期，其可溶性糖和可溶性蛋白的积累量得到显著增加，同时IAA和GA的含量也提高，这有效地平衡了插穗的内源激素水平，为不定根的生长提供了有利条件.服务把本文推荐给朋友加入我的书架加入引用管理器 E-mail AlertRSS作者相关文章魏尚霖黄嘉泳李永泉王溢Abstract：Prunus x blireana ‘Meiren’twigs cuttings were treated with 200mg/L ABT rooting agent,and water treatment was used as a control to compare and analyse the changes in external morphology,nutrient content, antioxidant enzyme activity and endogenous hormone content of the cuttings during rooting.The results showed that: (1) the overall time required for cuttings to form a large number of adventitious roots from the beginning of cutting was about 28 d; (2) ABT had a significant favourable effect on the rooting of cuttings; (3)the soluble sugars,soluble protcins，Proline(Pro)，growth hormone ( Indole-3-acetic Acid, IAA) and Gibberellins (GA) content were significantly higher than CK after the late stage of cutting; (4) for the trend of Superoxide Dismutase(SOD) activity，there was no significant difference between treatments and CK. Through the analyses, it can be concluded that the rooting of Prunus x blireana‘Meiren’requires high content of IAA and low content of GA,and higher levels of soluble sugars and soluble proteins in the carly stage can promote the induction and development of adventitious roots. The accumulation of soluble sugars and soluble proteins in ABT-treated cuttings was significantly increased in the middle and late stages of cutting, while the contents of IAA and GA were also increased, which effectively balanced the endogenous hormone levels of the cuttings and provided favourable conditions for the growth of adventitious roots.
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