白菜与甘蓝型油菜—萝卜F染色体附加系回交BC1中萝卜F染色体的精准鉴定
摘要：
参考萝卜染色体定位序列标记连锁图谱中F染色体连锁群的SSR分子标记,利用21个已知萝卜染色体组成的甘蓝型油菜—萝卜附加系材料,确定了萝卜F染色体特异的2个SSR标记RM-59-293和Rs1SSR3694-220,应用两个标记对白菜‘559’与甘蓝型油菜—萝卜F染色体附加系FF回交的BC1进行鉴定,筛选出10个含有萝卜F染色体的BC1单株。利用萝卜基因组特异探针pURsN对该10个含有萝卜F染色体的BC1单株的基因组进行荧光原位杂交,结果显示10个BC1均得到1个杂交信号,含有1条萝卜F染色体。进一步对该10个BC1单株进行主要农艺性状鉴定,筛选出形态接近白菜亲本‘559’的BC1-15单株和形态偏向白菜类的BC1-8、BC1-16、BC1-31和BC1-35单株。
关键词: 白菜, 附加系, 萝卜F染色体, SSR分子标记, 荧光原位杂交
Abstract:
Referring to the SSR molecular markers of chromosome F linkage group in the chromosome-assigned sequence-tagged linkage map of radish,two SSR molecular markers RM-59-293 and Rs1SSR3694-220 which were specific for radish chromosome F were confirmed by means of the known radish-chromosome information in the genetic background of 21 materials. Both SSR markers were used to identify the alien radish chromosome F in the genetic background of BC1 being derived from the backcross of Brassica rapa‘559’and rape-radish chromosome F addition line FF. As a result,10 BC1 plants added with alien radish chromosome F were obtained. Furthermore,the genome of the selected 10 BC1 plants with radish chromosome F were analyzed to detect the exogenous radish chromosome by fluorescence in situ hybridization technology using the radish genomic probe pURsN. The result showed that all of the 10 BC1 had one hybridization signal,which indicated that each of the tested BC1 plants was added with an exogenous radish chromosome. The main agronomic traits of the 10 BC1 plants were further identified. As a result,BC1-15 with the morphology similar to Chinese cabbage parent‘559’and BC1-8,BC1-16,BC1-31 and BC1-35 with the morphology partial to Chinese cabbage were selected.
Key words: Brassica rapa, additional line, radish chromosome F, SSR molecular marker, fluorescent in situ hybridization
中图分类号:
S634.1
S631.1
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