象耳豆根结线虫的PCR鉴定和检测方法
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象耳豆根结线虫的PCR鉴定和检测方法
龙海, 刘吴, 徐建华Development of a PCR Diagnostic for the Root-knot Nematode Meloidogyne enterolobii
LONG Hai, LIU Hao, XU Jian-huaAuthor information+
文章历史+
摘要
象耳豆根结线虫是一种在中国具有潜在经济重要性的农作物病原物。为提供有助于控制象耳豆根结线虫传播扩散的方法,研制了该线虫的快速PCR鉴定和检测法。该方法PCR引物的扩增目标为rDNA-IGS2区域,其设计依据象耳豆根结线虫与南方、爪哇、花生和北方根结线虫在该区域核酸序列的差异。通过对6种近似根结线虫的不同地理群体及自然土壤线虫群体的测试,验证了设计的PCR引物针对象耳豆根结线虫的特异性和可靠性。本方法具有快速灵敏的特点,可用于象耳豆根结线虫单条线虫的直接鉴定以及混合土壤线虫群体中象耳豆根结线虫的检测。
Abstract
The root-knot nematode Meloidogyne enterolobii Yang and Eisenback 1983 is a potentially important crop pathogen in China. To provide means to assist in preventing the spread of M. enterolobii, a single-step PCR diagnostic was developed for the nematode. The diagnostic primers were designed based on an alignment of ribosomal intergenic spacer 2 (IGS2) sequences from M. enterolobii, M. incognita, M. javanica, M. arenaria and M. hapla. The reliability of the diagnostic test has been validated by screening different geographic populations of six closely related Meloidogyne species and natural mixed soil nematode populations. The test is fast and sensitive, it can be used for direct diagnosis of a single nematode and for detection of M. enterolobii in mixed soil nematode populations.
关键词
象耳豆根结线虫 /IGS2序列 /PCR鉴定和检测法{{custom_keyword}} /
Key words
Meloidogyne enterolobii /IGS2 sequence /PCR diagnostic{{custom_keyword}} /
龙海, 刘吴, 徐建华.象耳豆根结线虫的PCR鉴定和检测方法[J]. 植物病理学报, 2006, 36(2): 109-115
LONG Hai, LIU Hao, XU Jian-hua.Development of a PCR Diagnostic for the Root-knot Nematode Meloidogyne enterolobii[J]. Acta Phytopathologica Sinica, 2006, 36(2): 109-115
中图分类号：Q959.172.5 S432.45
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脚注
{{custom_fn.content}}基金
This study was supported by grants from the National Natural Science Foundation of China (30471141) and the National "863" High-Tech Research Program (2001AA249021).
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