大花银莲花愈伤组织诱导及再生体系的建立
摘要： 为建立野生大花银莲花(Anemone silvestris)组培再生体系, 分别以无菌苗上、下胚轴、叶片和叶柄为外植体, 探讨不同浓度植物生长调节剂对不同外植体的愈伤组织诱导、不定芽分化、增殖与生根的影响。结果表明, 4种外植体均可诱导出不定芽, 其中上胚轴诱导效果最佳, 其在1/2MS+2.0 mg∙L-1 6-BA+0.1 mg∙L-1 NAA培养基中诱导率最高, 为86.67%; 最适增殖培养基为1/2MS+1.0 mg∙L-1 6-BA+0.05 mg∙L-1 NAA, 增殖系数为3.67; 最佳生根培养基为1/2MS+0.3 mg∙L-1 IBA, 生根率为100%; 在草炭:蛭石=2:1 (v/v)的栽培基质中, 组培苗的移栽成活率最高, 为98.33%。该研究有效解决了野生大花银莲花在园林及药用生产上的种质资源紧缺难题, 为工厂化育苗提供了技术支撑。
关键词: 大花银莲花, 愈伤组织, 植株再生, 组织培养
Abstract: In order to establish the tissue culture and regeneration system of wild Anemone silvestris, we investigated effects of different concentrations of plant growth regulators on callus induction, adventitious bud differentiation, proliferation and rooting of different explants including epicotyl, hypocotyl, leaf and petiole. The results showed that adventitious buds could be induced in all four explants, among them the epicotyl was the best. The best medium for hypocotyl induction was 1/2MS+2.0 mg∙L-1 6-BA+0.1 mg∙L-1 NAA with the highest induction rate of 86.67%; The optimal medium for proliferation was as follow 1/2MS+1.0 mg∙L-1 6-BA+0.05 mg∙L-1 NAA, and the proliferation coefficient was 3.67; The optimal rooting medium was 1/2MS+0.3 mg∙L-1 IBA with the rooting rate of 100%; In the medium of peat:vermiculite = 2:1 (v/v), the survival rate of tissue culture seedling was at 98.33%. This study effectively solved the shortage of germplasm resources of A. silvestris in garden and medicinal production, and provided technical support for the realization of factory seedling cultivation.
Key words: Anemone silvestris, callus, plant regeneration, tissue culture
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