柠条锦鸡儿遗传多样性研究.pdf
1、絮目蔗遗犬等博士学位论文柠条锦鸡儿遗传多样性研究聊73ZIOStudyongeneticdiversityofCaraganakorshinskiikom研究生：王赞指导教师：合作指导教师：申请学位门类级别：专业名称：研究方向：所在学院：韩建国教授高洪文研究员农学博士草业科学牧草种子、牧草育种及生物技术动物科技学院二零零五年六月本论文受国家“863”现代农业主题“抗旱耐盐碱生态环境建设用林灌木柠条、沙棘等新品种选育”(2002AA241
2、091)资助AbstractBasedonthenaturaldistributionofCaraganakorshinxkiiKonkinChinatenpopulationsofCkorshinskii(PGHQl，HQ2fmmShanxiprovince；SM，YLJBDBfromShaenxiprovince；EIK郴，DSfromInnerMongolia)wereselectedandinvestigatedGeneticd
3、iversities、Ⅳmevaluatedandcomparedatthreelevelofmorphology(i—eleaves，podsandseedsetc)，allozymeandDNAMainresultsobtainedaIeasfollows：(1)Molphologicaldiversitiesamong／withinpopulationswemdiscussedbyanalyzingcharacterssuchas
4、leavespo如’andseedsAnalysisofvarianceforallcharacteristicsw&ssignificantly础hemamongpopulationsandamongindividualswithinpopulationThemeanphenotypicdifferentiationcoefficient(Vsr=0195)showsthatthevariationamongpopulations(8
5、05％、issagnificanayhigherthanthatwithinpopulationsThecorrelationsbetweenthephenotypictraitsandecologicalandgeographicfactorsarenotsignificanL1000seedmass，podwidthandleaflengthwlglethemaintraimsaco)eotforthepheuotypicvaria
6、tionsaccoI【UngtothePCAanddiscimkmmmuaysi&BasedonthedusteranalysisandPeAthepopulationsofCkorshinskiiinvestigatedmaybedividedintofourgroupsbyphenotypictraits(2)Agelelectmphoresismethodwasusedtostudythegeneticdiversityoften
7、Ckorsh／ns蜮populationsEightof11locifromsevenenzymesassayedwerepolymorphiaCkorsh／nsk／／maintainedhighlevelofgeneticvariationascomparedwiththeaverageotherspeciesAtspecieslevelthenxannumberofallelesperlocus印)wasl727，themeanef
8、fectiveⅡu硼褂ofallelesperlocusa)wasl533，thepercentageof辨母妯。枇loci∞was727％，theebserveAk期∞蜀宫摅ityfito)吣0A25，the∞甲cctedlkmozygnsityQmw舔0292AtpopulationleveltheesliDulll强w讹』=1618A。群1470，P=618％，矗：=0408，and月：=0263The腑ngenedifferen
9、tiationcoefficient(G白)was014indicatingthatapproximately848％ofthetotalaBozymevariationoccurredwithinpopulationsThegeneflowamongpopulations帆)was15AccordingtotheUPGMAclusteranalysisbased∞thegeneticdistance，tenpopulationsofC
10、korgdns／d／wemdividedintothreegroupstherewasuosignificantrelationshipbetweengeneticdistanceandgeographicdistanceamongpopulations(3)ClearandhighresolutionDNAfingerprints、№mobtainedbySDSmethodTheoptimizedAFLPreactionsystemi
11、ncludeextractedgenomicD№《加0n砂q)wasdigestedwithEcoRlandMseIrestrictionenzymes；atotalvolumeof2Qulreactionmixture，thepreamplficationcousin2肛lofthedigesfon／Hgafionmixture，06芦leachofthepreamplificationptimers(50ng／／M])，犁lIOxP
12、CRbuffer1犁IMg“(25raM)，016一ldNWl“Ps(25raM)01犁lTaqpolymeruse(5U／／d)；Selectiveamplificationreactionmixture(2陬1)waspreparedwith犁ldilutedpreamplificationproduction，08meachEcoRIandMselprimera岫咖1)，犁l10xPCRbuffer121alM矿(2SmM)018
13、芦ldNTPs(25mM)o和lTaqpolymerase($U／p1)。Fourprimercombinationsptdiuced358bandsaczossatotalof98individualsHighgeneticdiversitywasbothobservedatspeA：icslevel(P=939％，日暑03168，I=0477)andpopulationslevel(Pj695％，H=0244，I=0365)Asig
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