何牡丹

花匠小妙招
2025-07-28 14:53

发现并阐明了决定鱼类卵质的新因子—Nanog及其作用的两个新机制：1）Nanog在早期胚胎中抑制母源β-catenin过度激活以保护胚胎发育；2）Nanog在卵子发生中通过“翻译控制”调控母源mRNA的翻译从而提升卵子质量。首次发现了鱼类胚胎中微同源末端连接（MMEJ）介导的DNA双链断裂修复占主导地位，并指导实现MMEJ介导的鱼类高效基因敲入。

代表性论文：

1，He, M.D., Zhang, R., Jiao, S.B., Zhang, F.H., Ye, D., Wang, H.P., and Sun, Y.H. (2020). Nanog safeguards early embryogenesis against global activation of maternal β-catenin activity by interfering with TCF factors. PLOS Biology 18, e3000561

2，He, M.D*., Jiao, S.B*., Zhang, R., Ye, D., Wang, H.P., and Sun, Y.H. (2022). Translational control by maternal Nanog promotes oogenesis and early embryonic development. Development 2022, 149(24)

3，He, M.D., Zhang, F.H., Wang, H.L., Wang, H.P., Zhu, Z.Y., and Sun, Y.H. (2015). Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos. Mutation Research 780, 86-96

4，Zhang, F.H., Li, X.M., He, M.D., Ye, D., Xiong, F., Amin, G., Zhu, Z.Y., and Sun, Y.H. (2020). Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells. Journal of Genetics and Genomics 47, 37-47

5，Ye, D., Wang, X.S., Wei, C.Y., He, M.D., Wang, H.P., Wang, Y.W., Zhu, Z.Y., and Sun, Y.H. (2019). Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b. PLOS Genetics 15, e1008306

6，Ye, D., Tu, Y.X., Wang, H.P., He, M.D., Wang, Y.Q., Chen, Z.F., Chen, Z.X., and Sun, Y.H. (2022). A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish. Water Biology and Security 1（3）10059

7，Zhang, F.H., Hao, Y.K., Li, X.M., Li, Y., Ye, D., Zhang, R., Wang, X.S., He, M.D., Wang, H.P., Zhu, Z.Y., and Sun, Y.H. (2021). Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation. Science China Life Science. 65, pages969–987

8，Sun, Y.H., Zhang, B., Luo, L.F., …… He, M.D. （2020） Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9. Genome Research 30, 118-126

9，Liu, C.J., Ye, D., Wang, H.P., He, M.D., and Sun, Y. H. (2020). Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study. Marine Biotechnology 22, 613-619.

10，Ye, D., Zhu, L., Zhang, Q.F., Xiong, F., Wang, H.P., Wang, X.S., He, M.D., Zhu, Z.Y., and Sun, Y.H. (2019). Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed by Lifetime Labeling of Germline. Marine Biotechnology (NY) 21, 217-228