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猫须草和石见穿的生药学对比研究.doc

花匠小妙招
2025-07-29 15:40

PAGE 猫须草和石见穿的生药学对比研究二级学院专业班级学生姓名学号指导教师目录摘要Ⅰ-ⅡTOC o 1-3 h u 29284 1前言 1 194 2 材料与仪器 2 6902 2.1 药材与试剂 2 20128 2.2 实验仪器 2 30278 3 方法与结果 2 6931 3.1 薄层色谱鉴别 2 12454 3.1.1展开剂的考察 3 29130 3.1.2提取溶剂的考察 4 23372 3.1.3提取方法的考擦 5 12055 3.1.4点样量的考察 6 9175 3.1.5提取时间的考察 6 23667 3.1.6预平衡和预饱和方式的考察 7 11476 3.1.7展开温度和湿度的考察 7 8455 3.1.8展开距离的考察 9 6337 3.1.9结果 9 1366 3.2 粉末显微鉴别 10 21371 3.2.1猫须草粉末性状 10 19085 3.2.2石见穿粉末性状 11 10567 4讨论 11 18419 参考文献 13 26449 综述 14 21316 致谢 20 PAGE PAGE I 猫须草和石见穿的生药学对比研究摘要：目的建立使用薄层色谱法及显微鉴别法对比区分猫须草和石见穿的的方法。方法对猫须草和石见穿进行薄层色谱、粉末显微鉴别的对比研究，采用单因素试验方法，筛选出最佳的薄层色谱条件；找出粉末显微特征。结果该薄层色谱鉴别条件吸附剂：硅胶G薄层板；展开剂：正己烷:乙酸乙酯:甲酸（7:6:0.4）；溶剂：甲醇；提取方法：超声处理30分钟；紫外光365nm下检视；石见穿粉末显微可观察到众多石细胞。结论所得薄层色谱斑点清晰，分离度高，重现性好，简便；显微镜下特征较容易分辨；可用于猫须草和石见穿的生药学对比研究。关键词：猫须草；石见穿；薄层鉴别；显微鉴别 PAGE PAGE III Clerodendranthus spicatus ?pharmacognosy comparative study of Salvia chinensis Abstract: Objective Using thin layer chromatography(TLC) and microscopic identification method pharmacognosy comparative study of Clerodendranthus spicatus and?Salvia chinensis method. Methods Clerodendranthus spicatus and?Salvia chinensis on thin layer chromatography, powder microscopic identification comparative study, by adopting the method of single factor experiment, selected the best thin layer chromatographic conditions. Find the powder microscopic characteristics. Results The TLC identification condition adsorbent: silica G thin layer plate; An agent: n-hexane: ethyl acetate: formic acid (7:6:0. 4); Solvent: methanol; Extraction methods: ultrasonic processing 30 minutes; Can be examined under a uv 365 nm；Salvia chinensis can be observed in many stone powder microscopic cells. Conclusion The TLC spots were clear, separating degree is high, good reproducibility, simple; Under a microscope, the characteristic is easy to distinguish, can be used in Clerodendranthus spicatus ?pharmacognosy comparative study of Salvia ch