白玉凤尾蕨孢子繁殖技术的研究
摘要： 研究了白玉凤尾蕨( Pteris cretica ‘Albo-lineata’) 孢子的无菌培养和常规繁殖方法。结果表明: 孢子萌发为书带蕨型, 配子体发育为水蕨型。1 /2MS培养基中萌发率最高, 可达82.3%; 低于2%的蔗糖浓度较适于孢子的萌发; MS培养基中配子体成苗率最高, 可达53.4%; 试管苗移栽成活率可达80%以上。草炭土+河沙(体积比1∶1) 是播种孢子的理想基质, 配子体成苗率达8912%; 当幼孢子体苗的第1片子叶长至1～2 cm长时分苗, 移栽到草炭土+蛭石+素砂+有机肥(体积比4∶2∶2∶1) 的栽培基质上,移栽成活率最高, 可达98.1%。幼孢子体苗长至10 cm左右时已经具有一定的观赏价值。
关键词: 凤尾蕨, 孢子, 无菌培养, 常规繁殖
Abstract: The spore sterile culture and routine propagation of Pteris cretica ‘Albo-lineata’were studied respectively. The results showed that the spore germination was of the Vittaria2type while the gametophyte development was of the Ceratop teris2type. The maximum germination rate was obtained in 1 /2MS medium, which reached 82.3%; the medium with sucrose concentration being less than 2% was appropriate for spore germination; the highest rate of gametophyte to sporophyte was achieved in MS medium, which reached
53.4%; the transplant survival rate of sporophyte in vitro reached above 80%. The mixed soil ( the turf and the sand blend in the volume ratio of 1 to 1) was the optimal growing medium for spore routine propagation, in which the rate of gametophyte to the sporophyte was up to 89.2%. When the first cotyledon of the sporophyte seedling grew to 1 - 2 cm, the sporophyte seedlings should be rep lanted in the growing medium which was composed of turf, vermiculite, sand and organic fertilizers. The highest survival rate reached 98.1%. The
young gametophyte seedlings of 10 cm height had already possessed ornamental values.
Key words: Pteris cretica, Spore, Routine propagation, Sterile culture
中图分类号:
S 682.35
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