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裸花紫珠试管苗生产技术初探

             [    ]                    WORD,      。   :         (Callicarpa nudiflora Hook. et Arn.)       ,         。    , 0.1%        15 min,    MS+1.5 mg/L 6-BA+30 g/L      ,7 d     ;    MS+2.0 mg/L 6-BA+30 g/L            ,     8.50;           30 d,  40 d       ;   MS+0.5,0.8 mg/L NAA+0.5 g/L   +30 g/L        ,    100%;      V  ?V  ?V  =1?1?1      ,      20 d    93%,       。    :    (Callicarpa nudiflora Hook. et Arn.);   ;  ;     S567.1+9;Q943.1 A 0439-8114(2014)13-3121-03 Production Technologies of Callicarpa nudiflora Hook. et Arn. Test-Tube Plantlets HUANG Sai,PAN Mei,QI Hua-sha,WANG Jing-fei,L,, De-ren (Institute of Horticulture and Flower, Hainan Academy of Agricultural Sciences, Haikou 571100,    www.taodocs.com                    [    ]                    WORD,      。   :         (Callicarpa nudiflora Hook. et Arn.)       ,         。    , 0.1%        15 min,    MS+1.5 mg/L 6-BA+30 g/L      ,7 d     ;    MS+2.0 mg/L 6-BA+30 g/L            ,     8.50;           30 d,  40 d       ;   MS+0.5,0.8 mg/L NAA+0.5 g/L   +30 g/L        ,    100%;      V  ?V  ?V  =1?1?1      ,      20 d    93%,       。    :    (Callicarpa nudiflora Hook. et Arn.);   ;  ;     S567.1+9;Q943.1 A 0439-8114(2014)13-3121-03 Production Technologies of Callicarpa nudiflora Hook. et Arn. Test-Tube Plantlets HUANG Sai,PAN Mei,QI Hua-sha,WANG Jing-fei,L,, De-ren (Institute of Horticulture and Flower, Hainan Academy of Agricultural Sciences, Haikou 571100,    www.taodocs.com       

China) Abstract: Using tender stem segments of Callicarpa nudiflora as explants, the techniques of tissue culture and rapid propagation in vitro were studied. Results showed that the optimum disinfector was 0.1% HgCl2 for 15 min, with MS+6-BA1.5 mg/L+ sucrose 30 g/L as the initial medium. After inoculation for 7 d, the axillaries buds began to germinate. Using MS+6-BA 2.0 mg/L + sucrose 30 g/L as the proliferation medium, the proliferation multiples reached 8.50. The best cycle of subculture training was 30 d. The plantlets would appear dead when more than 40 d. The rooting culture medium was MS+NAA 0.5,0.8 mg/L+ active carbon 0.5 g/L + sucrose 30 g/L,with the best rooting effect and the rate of rooting of 100%. When the rooted seedlings were transplanted into the mediums of sand?soil?sawdust(1?1?1) and covered with plastic film for keeping moisture for 20 d, the survival rate reached up to 93%. Key words: Callicarpa nudiflora Hook. et Arn.; test-tube plantlet; stem segment; tissue culture     (Callicarpa nudiflora Hook. et Arn.)          ,             。        、  、  、  、  、  、  、  、  、    ,           [1]。     www.taodocs.com       China) Abstract: Using tender stem segments of Callicarpa nudiflora as explants, the techniques of tissue culture and rapid propagation in vitro were studied. Results showed that the optimum disinfector was 0.1% HgCl2 for 15 min, with MS+6-BA1.5 mg/L+ sucrose 30 g/L as the initial medium. After inoculation for 7 d, the axillaries buds began to germinate. Using MS+6-BA 2.0 mg/L + sucrose 30 g/L as the proliferation medium, the proliferation multiples reached 8.50. The best cycle of subculture training was 30 d. The plantlets would appear dead when more than 40 d. The rooting culture medium was MS+NAA 0.5,0.8 mg/L+ active carbon 0.5 g/L + sucrose 30 g/L,with the best rooting effect and the rate of rooting of 100%. When the rooted seedlings were transplanted into the mediums of sand?soil?sawdust(1?1?1) and covered with plastic film for keeping moisture for 20 d, the survival rate reached up to 93%. Key words: Callicarpa nudiflora Hook. et Arn.; test-tube plantlet; stem segment; tissue culture     (Callicarpa nudiflora Hook. et Arn.)          ,             。        、  、  、  、  、  、  、  、  、    ,           [1]。     www.taodocs.com       

  、  、       ,      、    、    、    、     、      [2,3]。             ,              ,             ,           ,             。                ,           ,         。               、       [4-7],         [8,9],  ,                          。 1       1.1                            。 1.2    1.2.1                    ,         ,        75%     10 s,   0.1%      15 min,      5          。   ,           ,   MS+1.5 mg/L 6-BA    。       ,           。 1.2.2      MS      ,                   。        30 g/L  (          ),7 g/L    ,pH 5.8。        121 ?,0.14 MPa  20 min[10]。    www.taodocs.com         、  、       ,      、    、    、    、     、      [2,3]。             ,              ,             ,           ,             。                ,           ,         。               、       [4-7],         [8,9],  ,                          。 1       1.1                            。 1.2    1.2.1                    ,         ,        75%     10 s,   0.1%      15 min,      5          。   ,           ,   MS+1.5 mg/L 6-BA    。       ,           。 1.2.2      MS      ,                   。        30 g/L  (          ),7 g/L    ,pH 5.8。        121 ?,0.14 MPa  20 min[10]。    www.taodocs.com       

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