珍珠梅组培快繁体系的建立
摘要： 以珍珠梅茎段为外植体,研究不同消毒时间、不同培养基、激素种类和浓度对珍珠梅离体培养的影响,建立了珍珠梅组培快繁体系,结果发现：用2%有效氯次氯酸钠,消毒8 min,接种茎段成活率最理想;使用培养基WPM+GA3 5 mg/L作为初代培养,腋芽萌发率极高,平均生长量长为6.01 cm,生长效果最为理想;培养基MS+BA 0.5 mg/L+IAA 1.0 mg/L作为继代增殖培养基,培养30 d后增殖系数是8.13,效果最佳;生根培养基条件效果最佳为1/2 MS+NAA 0.5 mg/L;试验中将V草炭∶V蛭石∶V珍珠岩=1∶1∶1作为移栽基质,可达到理想的移栽效果,在保证根系完整的情况下,30 d后调查移栽成活率可达到90%以上。
关键词: 珍珠梅, 快繁, 茎段, 腋芽
Abstract: In order to establish a rapid propagation system for tissue culture of Sorbaria sorbifolia,in this study the stem segments of Sorbaria sorbifolia were used as explants to study the effects of different disinfection times, different basic media, hormone types and concentrations on in vitro culture of Sorbaria sorbifolia. The results showed that using a 2% effective chlorine sodium hypochlorite solution for disinfection for 8 minutes and inoculating the stem segment disinfectant had the most ideal effect; using WPM+GA3 5 mg/L as the primary culture medium, the germination rate of axillary buds was the highest, with an average growth length of 6.01 cm, and the growth effect was the most ideal; using MS+BA 0.5 mg/L+IAA 1.0 mg/L as the proliferation medium, the proliferation coefficient reached 8.13 after 30 days of cultivation, indicating the best effect; the optimal rooting medium is 1/2 MS+NAA 0.5 mg/L; using peat∶vermiculite∶perlite=1∶1∶1（v/v）as the transplant substrate, the survival rate of transplantation can reach over 90% after 30 days, while ensuring the integrity of the root system.
Key words: Sorbaria sorbifolia, rapid propagation, stem segment, axillary bud
中图分类号:
S685
相关知识
珍珠梅组培快繁体系的建立
三种野生姜科花卉组培快繁体系的建立
泼墨石斛高位芽组培快繁体系的建立
‘白天堂’百合的组培快繁体系的建立
凤梨释迦组培快繁体系的建立
蝴蝶兰组培快繁技术
酸樱桃组培快繁技术体系的研究
猕猴桃组培快繁育苗专利技术资料文集
大花天竺葵的组培快繁技术体系研究.pdf
植物组培快繁彩图详解
网址: 珍珠梅组培快繁体系的建立 https://m.huajiangbk.com/newsview2436611.html
| 上一篇: 珍珠梅的繁殖与管理 |
下一篇: 【花艺】一日一花-珍珠梅 |
