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肉苁蓉提取工艺及干燥方式研究

花匠小妙招
2026-06-01 07:05

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[摘要]

目的选择肉苁蓉最佳提取工艺参数，研究不同干燥方式对5种苯乙醇苷类成分的影响。方法采用单因素筛选结合Box-Behnken响应面法优化提取工艺。最优条件提取后，利用HPLC法检测不同干燥方式肉苁蓉的松果菊苷、肉苁蓉苷A、毛蕊花糖苷、异毛蕊花糖苷、2'-乙酰基毛蕊花糖苷成分含量并采用单因素方差分析、聚类分析、主成分分析、密切值法分析5种苯乙醇苷类成分含量，选择最佳干燥方式。结果最佳提取工艺：甲醇体积分数55.14%，液料比46.39，提取时间38.50 min。聚类分析、主成分分析、密切值法分析表明，采用冷冻干燥法所得到的肉苁蓉品质最好，80℃烘干次之，40℃烘干最低。结论使用该工艺提取肉苁蓉，其5种苯乙醇苷类成分提取完全、充分。虽然冷冻干燥法炮制肉苁蓉所保留有效成分最高，但从生产角度考虑，采用80℃烘干法炮制能达到成本和功效的平衡。

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[Abstract]

Objective The optimum extraction process parameters of Cistanche deserticola were selected to study the effects of different drying methods on five phenylethanoid glycosides. Methods Single factor screening combined with Box-Behnken response surface method was used to optimize the extraction process. After optimal conditions were extracted, HPLC method was used to detect the content of echinacoside, cistanche A, verbascoside, isoacteoside, and 2'-acetylacteoside in different drying methods, and one-way ANOVA, cluster analysis, principal component analysis and close value analysis were used to analyze the content of five phenylethanoid glycosides to choose the best drying method. Results Optimal extraction process was as following:methanol volume fraction was 55.14%, liquid to material ratio was 46.39, extraction time was 38.50 min. Cluster analysis, principal component analysis, and close value analysis showed that the quality of C. deserticola obtained by freeze-drying method was the best, followed by drying at 80℃ and the lowest at 40℃. Conclusion Using this process to extract C. deserticola, the five phenylethanoid glycosides are completely and fully extracted. Although the freeze-drying method of C. deserticola has the highest active ingredient retention, from the production point of view, the 80℃ drying method can achieve a balance of cost and efficiency.

[基金项目]

国家自然科学基金资助项目（31860349）；国家自然科学基金资助项目（31860102）；甘肃省科技计划资助项目（18YF1NA072）