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码上扫一扫！外源ABA对仁用杏花期抗寒力及相关生理指标的影响魏安智1, 杨途熙1, 张睿1西北农林科技大学生命科学学院摘要: [目的]探讨外源ABA对仁用杏花期抗寒力的影响.[方法]以8年生仁用杏为材料,自来水为对照,在仁用杏花芽膨大期喷施18 mg/L ABA后,分别测定花蕾、花朵和幼果的抗寒力及相关生理指标.[结果]与对照相比,花芽膨大期喷施18 mg/L ABA,可降低仁用杏花蕾,花朵和幼果的电解质渗出率,且在较低温度下效果尤其显著;可使半致死温度分别降低2.73,1.73和0.38℃;MDA含量分别降低37.76%,55.1%和45.41%;SOD酶活性分别提高2.3%,9.9%和0.7%;POD酶活性分别提高9.0%,0.7%和3.4%;可溶性糖含量分别提高6.5%,15.24%和12.3%;内源ABA含量分别提高275%,61.7%和8.3%;使ABA/GA3.维持较高水平.[结论]花芽膨大期喷施18 mg/L ABA,可在不影响仁用杏花蕾、花朵和幼果发育进程的情况下,提高仁用杏花期的抗寒力.关键词: ABA 仁用杏花期抗寒力 DOI：分类号: 基金项目:国家“十一五”科技支撑项目（2006BAD18B0201）；陕西省重大科技专项计划项目(2006kz09-G6） Effect of exogenous ABA on cold-resistant ability and related physiological indicators of almond-apricot during blossom period Abstract: 【Objective】 This paper studied the effects of exogenous ABA on cold-resistance of almond-apricot during blossom period.【Method】 Taking 8-year-old almond-apricot as the material,running water as comparison,the cold-resistance and related physiological targets of the flower bud,flowers and young fruit's were determined respectively after spraying 18 mg/L ABA during the swelling-time of flower bud.【Result】 Compared to control,the results showed that the treatment could decrease the electrolyte osmotic rates of flower buds,flowers and young fruits,especially in lower temperature.It could also decrease the semi-lethal temperature by 2.73,1.73,0.38 ℃ and MDA content by 37.76%,55.1%,45.41% respectively,while the activity of SOD was enhanced by 2.3%,9.9%,0.7% and the activity of POD was enhanced by 9.0%,0.7%,3.4% respectively.Soluble sugar was increased by 6.5%,15.24%,12.3% and endogenous ABA content was increased by 275%,61.7%,8.3% respectively.The treatment could keep a higher ratio of ABA/GA3.【Conclusion】 Spurting 18 mg/L ABA during the swelling-time could enhance the cold-resistance of almond-apricot during blossom period in the situation of not affecting flower bud,flowers and young fruit development of almond-apricot. Key words: ABA almond-apricot blossom period cold-resistant ability 参考文献(共18条):[1]彭艳华,黄永秀.脱落酸应答基因的结构、表达调控及信号转导[J].植物生理学通讯,1996,32(2):155-158.Peng Y H,Huang Y X.Structure.expression regulation and signal transduction of ABA-responsive gene[J].Plant Physiology Communications,1996,32(2):155-158.(in Chinese)[2]谢吉容,向邓云,梅虎,等.南方红豆杉抗寒性的变化与内源激素的关系[J].西南师范大学学报:自然科学版,2002,27(2):231-234.Xie J R,Xiang D Y,Mei H,et al,Relationship between changes of cold resistance and endogenous hormone of Taxus chinensis var.mairei Chenget L.K.F[J].Journal of Southwest China Normal University:Natural Science Fatition,2002,27(2):231-234.(in Chinese)[3]郭凤领,卢育华,李宝光.外源ABA对番茄苗期和开花期抗冷特性的影响[J].山东农业大学学报:自然科学版,2000,31(4):357-362.Guo F L,Lu Y H,Li B G.Effect of exogenous ABA on chilling-resistance during seedling and floreseencein tomato[J].Journal of Shandong Agricultural University:Natural Science Edition,2000,31(4):357-362.(in Chinese)[4]党秋玲,余超,王祯丽.ABA处理种子对加工番茄幼苗抗寒力及相关生理指标的影响EJ].石河子大学学报;自然科学版,2005.23(3):349-351.Dang Q L,Yu C,Wang Z L.Studies on the chilling resistanceand physiological index in processing tomatoesafter treatingseeds by ABA[J].Journal of Shihezi University:Natural Sci-ence Edition,2005,23(3):349-351.(in Chinese)[5]曾韶西,王以柔,李美茹.等.冷锻炼和ABA诱导水稻幼苗提高抗冷性期间膜保护系统的变化[J].热带亚热带植物学报,1994,2(I):44-50.Zeng S X,Wang Y R,Li M R,et al.Change of the membraneprotective system during the enhancement of chilling resistanceinduced by cold hardening and ABA treatment in rice seedlings[J].Tropical Subtropical Botany,1994,2(1):44-50.(in Chi'nese)[6]李亚男,陈大清,胡培丽.ABA和6-BA对不同温度条件下芝麻幼苗某些生理指标的影响[J].长江大学学报:自然科学版,2005,2(8):67-68.Li Y N,Chen D Q,Hu P L.Effects of ABA and 6-BA on somephysiological characteristics of sesame under different tempera-tures[J].Journal of Yangtze University:Natural Science Edi-tion.2005,2(8):67-68.(in Chinese)[7]杨向娜,魏安智,杨途熙,等.仁用杏3个生理指标与抗寒性的关系研究[J].西北林学院学报,2006,21(3):30-33.Yang X N.Wei A Z,Yang T X,et al.Studies on relationshipsbetween soluble protein contents,SOD and POD activity and cold resistant ability of 3 apricot varieties[J].Journal of Northwest Forestry University,2006,21(3):30-33.(in Chinese)[8]龚月桦,周永学,樊军锋,等.美国黄松、班克松和油松的抗寒性比较[J].应用生态学报,2006,17(8):1389-1392.Gong Y H,Zhou Y X,Fan J F,et al.Cold hardiness of Pinus ponderosa,P.banksian and P.tabulaeforrnis[J].Chinese Journal of Applied Ecology,2006,17(8):1389-1392.(in Chinese)[9]高俊凤.植物生理学实验技术[M].西安:世界图书出版公司,2000.
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