广谱裂解性耐药沙门氏菌噬菌体的分离筛选及其在食品安全中的应用

• 花匠小妙招
• 2024-11-12 05:19

声明

Table of content

摘要

ABSTRACT

LIST OF ABBREVIATIONS

Chapter 1 Review of literature

1.1Salmonellosis and its association with poultry

1.2Antibiotic-resistant Salmonella in poultry

1.3The rise of antibacterial resistance

1.4Phage biology and history

1.5Cleavage pathway oflysogenic phage and lytic phage

1.6Phage taxonomy based on morphology and genome composition

1.7Phage selection criteria for biocontrol and therapy

1.8Phages an alternative to antibiotics in controlling pathogens

1.9Bacteriophages in Industrial Applications

1.10Bacteriophage in pharmaceutical formulations

1.11Phages perform as biocontrol and therapeutic tools

1.12 Application of phages for controlling Salmonella infections in poultry and poultry products

1.13Phages against biofilms

1.14Phages for detection of bacterial pathogens

1.15Safety evaluation of phages

1.16Commercial phage products

1.17Study proposal

Chapter Ⅱ Isolation and characterization of phage ISTP3 for biocontrol application against drug-registrant Salmonella contamination on foods

2.1Introduction

2.2Materials and Methods

2.2.1Test materials and reagents

2.2.2Test equipments

2.2.3Bacterial strains

2.2.4Bacteria strains and growth conditions

2.2.5Isolation, propagation and purification of phage

2.2.6Spot Test

2.2.7Efficiency of plating

2.2.8Transmission electron microscopy (TEM)

2.2.9Absorption Rate

2.2.10One-step growth curves of phage ISTP3

2.2.11Thermal and pH stability of phage

2.2.12Phage Sequencing, Genome Annotation and Comparison

2.2.13Biological control of drug resistant S. enterica in foods

2.2.14Statistical analysis

2.3Results

2.3.1Phage isolation and characterization

2.3.2Characteristics of phage ISTP3

2.3.3Relative replication efficiency of phage ISTP3

2.3.4Genome Sequencing and bioinformatics analysis

2.3.5 Evaluating the efficacy of phage ISTP3 to reduce the drug resistantS. Typhimurium in foods

2.4Discussion

Chapter Ⅲ Conclusions, innovations of the study and future

3.1Conclusions

3.2Innovations of the present study

3.3Future direction

References

Acknowledgements

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