西花蓟马抗噻虫胺种群对杀虫剂的交互抗性及机制
摘要： 噻虫胺是具有内吸和触杀等多种作用方式的新烟碱类杀虫剂,常用于防治入侵害虫西花蓟马。为明确抗性风险,本文研究了西花蓟马抗噻虫胺种群对多种杀虫剂的交互抗性及其机制。经过45代筛选,西花蓟马对噻虫胺产生了高水平抗性(56.8倍)。生物测定结果表明: 西花蓟马高抗噻虫胺种群与噻虫嗪、吡虫啉、毒死蜱、三氟氯氰菊酯、甲维盐存在中等水平交互抗性(18.6>RR50>11.3),对辛硫磷及灭多威具有低水平交互抗性,与溴虫腈和多杀菌素不存在交互抗性。胡椒基丁醚(PBO)与磷酸三苯酯(TPP)对杀灭西花蓟马抗噻虫胺种群(CL)、云南田间种群(YN)和敏感种群(S)均有显著增效作用。西花蓟马抗噻虫胺种群细胞色素P450含量(3.6倍)、细胞色素b5含量(2.9倍)及O-脱甲基酶活性(4.9倍)和羧酸酯酶活性(2.5倍)均显著高于敏感种群,表明多功能氧化酶及羧酸酯酶活性增强是西花蓟马对噻虫胺产生抗性的重要机制。
关键词: 西花蓟马, 噻虫胺, 多杀菌素, 交互抗性, 抗性机制
Abstract: Clothianidin, belonging to neonicotinoid insecticide with systemic and contact mechanisms, is used to control the invasive pest Frankliniella occidentalis. To identify the resistance risk, we examined the cross-resistance to multiple insecticides and mechanisms of clothianidin resistant population of F. occidentalis. The results showed that F. occidentalis developed a high level of resis-tance to clothianidin (56.8-fold) after selecting for 45 generations. The resistant population of F. occidentalis had medium level of cross-resistance to thiamethoxam, imidacloprid, chlorpyrifos, cyhalothrin and emamectin benzoate (18.6>RR50>11.3), and the low level of cross-resistance to phoxim and methomyl, but no cross-resistance to chlorfenapyr and spinosad. The synergists piperonyl butoxide (PBO) and triphenyl phosphate (TPP) had significant synergistic effects on clothianidin in killing the resistant population (CL), Yunnan wild population (YN) and susceptible population (S). Compared with the sensitive population, the CL populations had significantly increased activities of mixed-functional oxidases P450(3.6-fold), b5(2.9-fold) and O-demethylase (4.9-fold), and carboxylesterase (2.5-fold), with no significant difference in the activities of glutathione S-transferases among CL and S populations. The results highlight the role of increasing mixed-functional oxidases and carboxylesterase in the resistance of F. occidentalis to clothianidin.
Key words: Frankliniella occidentalis, clothianidin, spinosad, cross-resistance, resistance mechanism
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