番茄花叶病毒番茄分离物与烟草花叶病毒蚕豆分离物生物学、血清学比较及PCR特异性检测
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番茄花叶病毒番茄分离物与烟草花叶病毒蚕豆分离物生物学、血清学比较及PCR特异性检测
周雪平, 薛朝阳, 刘勇, 李德葆BIOLOGY AND SEROLOGICAL COMPARISON OF TOMV TOMATO ISOLATE AND TMV BROAD BEAN ISOLATE AND SPECIFIC DETECTION WITH POLYMERASE CHAIN REACTION
Zhou Xueping, Xue Chaoyang, Liu Yong, Li DebaoAuthor information+
文章历史+
摘要
对番茄花叶病毒番茄分离物(ToMV-To-S1)和烟草花叶病毒蚕豆分离物(TMV-B935A)进行了生物学和血清学比较。人工接种6科20种植物,这两个分离物均能侵染其中的3科14种植物,但在普通烟(白肋烟、黄苗榆)上的症状差异较大,前者在这些植物上引起局部症状,后者为系统症状。在琼脂双扩散试验中,两者与ToMV-To-S1抗血清和4种TMV抗血清均形成沉淀线,但沉淀线之间产生刺角,说明ToMV-To-S1和TMV-B935A虽有血清关系,但亦有差异。胶内交叉吸附试验也证明两者之间有血清学差异。根据已报道的ToMV-L株系和TMVU1株系CP基因的3'端非编码区序列设计了ToMV和TMV特异引物,采用PCR方法,这些引物能够特异性的检测区分ToMV-To-S1和TMV-B935A。
Abstract
Biology and serological comparison between ToMV tomato isolate (ToMV-To-S1) and TMV broad bean isolate (TMV-B935A) was made. Both ToMV-To-S1 and TMV-B935A could infected 14 plant species in 3 families when 20 plant species in 6 families were tested. The difference was found in Nicotiana tabacum (N. tabacum-White Burley, N. tabacum-Huang Miaoyu), ToMV-To-S1 induced local lesions in these plant species, while TMV-B935A produced systemic mosaic symptoms. In double-diffusion tests, spur formation was observed between ToMV-To-S1 and TMV-B935A, and the results of intragel cross-absorption tests agreed with the doublediffusion tests results. Specific primers corresponding to the coat protein gene and 3' non coding region were designed. When ToMV-To-S1 cDNA templete were used, a specific band could be amplified with ToMV primers by PCR, No band was found when we used TMV primers. With TMV-B935A templete, only TMV primers could produce a specific band. So it is easy to distinguish ToMV-To-S1 and TMV-B935A with ToMV and TMV specific primers.
关键词
番茄花叶病毒番茄分离物 /烟草花叶病毒蚕豆分离物 /生物学 /血清学 /PCR检测{{custom_keyword}} /
Key words
ToMV-To-S1 /TMV-B935A /Biology /Serology /Detection by PCR{{custom_keyword}} /
周雪平, 薛朝阳, 刘勇, 李德葆.番茄花叶病毒番茄分离物与烟草花叶病毒蚕豆分离物生物学、血清学比较及PCR特异性检测[J]. 植物病理学报, 1997, 27(1): 53-58
Zhou Xueping, Xue Chaoyang, Liu Yong, Li Debao.BIOLOGY AND SEROLOGICAL COMPARISON OF TOMV TOMATO ISOLATE AND TMV BROAD BEAN ISOLATE AND SPECIFIC DETECTION WITH POLYMERASE CHAIN REACTION[J]. Acta Phytopathologica Sinica, 1997, 27(1): 53-58
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