黄龙病菌和植原体在长春花体内的移动、增殖以及相关症状研究
由“Candidatus Liberibacter asiaticus”(CLas)引起的柑橘黄龙病是我国柑橘生产上最严重的病害之一。有研究发现,感染柑橘黄龙病的甜橙植株内可以同时检测到CLas和相关植原体(“Candidatus Phytoplasma asteri”,CPa),但二者关系尚不清楚。本研究利用长春花为实验材料,采用不同嫁接组合探究CLas与CPa的互作关系。研究结果表明:CPa单独接种长春花造成花变叶和节间缩短症状,但不会致长春花死亡;CLas单独接种长春花产生叶片斑驳症状,接种后2个月长春花死亡。CLas和CPa同时接种长春花,出现斑驳伴随花变叶症状,长春花约3个月后死亡,相比CLas单独接种,长春花的存活时间更长。研究结果还表明CLas在长春花植株内的转移速率较CPa快,但增殖速率较CPa慢。本研究通过人工接种CLas和CPa,分析2种接种体在长春花植株上的症状表现,以期为研究CLas与CPa在长春花上的症状表现差异及其互作关系提供理论依据。
Abstract
Citrus Huanglongbing, caused by "Candidatus Liberibacter asiaticus" (CLas), is one of the most destructive diseases in citrus production. Early studies showed that CLas and phytoplasma ("Candidatus Phytoplasma asteri", CPa) can be simultaneously detected in CLas-infected citrus, however, the relationship between CLas and CPa remains unknown. In this study, periwinkle (Catharanthus roseus) was used as host materials to explore the relationship between CLas and CPa by different grafting combinations. The result revealed that the periwinkle plant showed the phyllody and shortened internode when only infected with CPa, but didn’t cause the death of plant. The CLas-infected periwinkle showed mottled leaves and died in 2 months after grafting. When the periwinkle was infected with CLas and CPa, the plant showed phyllody along with mottled leaves and died in about 3 months after grafting, however, this was longer than the survival time of periwinkle only infected with CLas. In addition, our results showed that the movement speed of CLas was faster than CPa, but the propagation of CLas was slower than CPa. This study provided a theoretical basis for studying the difference of symptoms caused by CLas and CPa and interaction between CLas and CPa in periwinkle.
关键词
黄龙病菌 /植原体 /长春花{{custom_keyword}} /
Key words
Candidatus Liberibacter asiaticus /Candidatus Phytoplasma asteri /Catharanthus roseus{{custom_keyword}} /
陈俊甫, 郑正, 邓晓玲.黄龙病菌和植原体在长春花体内的移动、增殖以及相关症状研究[J]. 植物病理学报, 2019, 49(2): 160-166 https://doi.org/10.13926/j.cnki.apps.000374
CHEN Jun-fu, ZHENG Zheng, DENG Xiao-ling.Research on the movement and propagation of "Candidatus Liberibacter asiaticus" and "Candidatus Phytoplasma asteri" in periwinkle and their associated symptoms[J]. Acta Phytopathologica Sinica, 2019, 49(2): 160-166 https://doi.org/10.13926/j.cnki.apps.000374
参考文献
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脚注
{{custom_fn.content}}基金
国家现代农业(柑橘)产业技术体系(CARS-26)
{{custom_fund}}相关知识
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