哈密瓜种质资源苗期细菌性果斑病抗性鉴定
摘要： 【目的】 筛选出高抗细菌性果斑病的甜瓜资源,为选育抗病品种奠定基础。【方法】 采用室内苗期人工喷雾法接种,采用2种抗性分级方法,以感病品种炮台红为对照,对17份甜瓜资源进行抗性鉴定。【结果】 17份供试甜瓜材料中,没有对细菌性果斑病菌免疫的材料,JM17-14的抗性最强,相对抗病指数为0.85,属高抗水平;采用两种抗性分级标准,供试甜瓜材料的抗性强弱顺序基本一致,但抗性水平有差异;17份供试材料中杂交F1代的抗性多介于其双亲之间,但也有超亲优势存在。【结论】 17份供试甜瓜材料中,JM17-14的抗性最强,为高抗细菌性果斑病资源,可用于抗性基因定位及抗病品种选育。
关键词: 哈密瓜; 细菌性果斑病; 抗性鉴定; 苗期接种
Abstract: 【Objective】 The occurrence of bacterial fruit spot disease of Hami melon in Xinjiang is increasing year by year. This project aims to lay a foundation for the screening of cantaloupe resources with high resistance to bacterial fruit spot disease and the breeding of resistant varieties.【Method】 In this study, the susceptible variety "Bataonghong" as control, artificial spray inoculation and two resistance classification methods were used to identify the resistance to Acidovoraxavenae subsp. Citrulli among 17 cantaloupe cultivars at the seedling stage. 【Result】 Indoor inoculation test indicated that the resistance of JM17-14 was the strongest among the 17 test materials. The relative disease-resistance index was 0.85, which expressed high resistance level. When using two resistance classification criteria, the resistance order of the tested melon was basically the same, but the resistance level was different, and the resistance of F1 hybrids in 17 tested materials was mostly between their parents, but there was also overparent dominance.【Conclusion】 JM17-14 is the resource of high resistance to bacterial fruit spot disease among the 17 tested melon materials, which can be used for the resistance gene localization and the breeding of disease-resistant varieties. The resistance level of the same melon variety resource is different by using different resistance grading standards. For this purpose, uniform resistance grading standard is required and the results of different investigators are more valuable. The resistance of Hami melon to Acidovoraxavenae subsp. Citrulli may be controlled by multiple genes, and the resistance genetic mechanism needs further study.
Key words: cantaloupe; bacterial fruit spot disease; resistance identification; seedling inoculation
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