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茶树花黄酮类物质抗肿瘤作用研究

花匠小妙招
2024-11-28 17:55

摘要: 为促进茶树产业发展,提取茶树花黄酮类物质探讨其抗肿瘤作用。在96孔板中加入MCF-7细胞悬液,37℃5%CO2培养24h后分别加入25μL培养基1640、蒸馏水、茶树花0.5c黄酮提取物溶液进行MTT Assay。在6孔板中加入MCF-7细胞悬液37℃5%CO2培养至细胞铺满培养板,分别加170μL培养基1640、蒸馏水、0.5c黄酮粗提物溶液进行Scratch Assay。结果显示:在MTTAssay中茶树花黄酮粗提物溶液浓度越高细胞的存活率越低（0.5c>0.25c>0.125c）。Scratch Assay中在空白对照组（蒸馏水）和阴性对照组（培养基1640）MCF-7细胞刻痕间距逐渐缩短且变化较大;在0.5c黄酮粗提物溶液处理组MCF-7细胞刻痕间距几乎没变化。研究表明茶树花中的黄酮类物质能较强地抑制癌细胞MCF-7的生长。

关键词: 茶树花; / 黄酮; / 抗肿瘤;

Abstract: In order to promote the development of the tea industry, the flavonoids in the tea flowers were extracted and its anti-tumor effect was investigated. First, MCF-7 was dropped into 96 well plates and cultured at 5% CO 2 , 37℃ in 24 hours. 25μL medium 1640, 25μL distilled water, 25μL 0.5c flavonoids extract solution from the tea flower was respectively added to MTT Assay. Next, MCF-7 was dropped into 6 well plates and cultured to the full well at 5% CO 2 , 37℃ . 170μL medium 1640, 170μL distilled water, 170μL 0.5c flavonoids crude extract solution was respectively added to Scratch Assay. The results showed that in MTT Assay, the higher the flavonoids extract solution concentration was, the lower the cell survival rate was, that was to say, the cell survival rate was 0.5c>0.25c>0.125c. In Scratch Assay, MCF-7 cell scratch space at the blank control group (medium 1640) and negative control group (distilled water ) gradually shortened and varied greatly , but the 0.5c flavonoids extract solution′ s was almost no change. The study suggested that the flavonoids in the tea flower could strongly inhibit the MCF-7 growth.

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