CaMV35S启动子及其在转基因作物中的应用和检测
摘要： 花椰菜花叶病毒35S启动子(CaMV35S)是植物基因工程中应用最广泛的启动子之一,它在转基因植物的安全评价及检测研究中具有重要意义。了解CaMV35S启动子的起源及其发展情况,是开展转基因安全评价和检测的前提。文章对CaMV35S启动子的发展历史及其结构、功能作了简要描述,分析总结了CaMV35S启动子在转基因作物中的应用情况和目前对其相应的检测方法。针对目前在转基因作物中检测CaMV35S启动子存在的问题,认为今后转基因相关作物的序列信息及检测数据需要交流和共享,同时各个检测机构或实验室之间需要展开数据共享与共同验证,从而建立针对CaMV35S启动子的相对统一的标准检测方法。
关键词: CaMV35S启动子, 转基因作物, 增强子, PCR
Abstract: CaMV35S promoter is one of the most important promoter that widely used in plant genetic engineering. It plays a key role in the safety evaluation and detection of transgenic plants. Understanding the origin and development of CaMV35S promoter is the prerequisite for carrying out safety assessment and detection of transgenic plants. In this review, history of CaMV35S promoter and its structure and function were briefly described. Then, application of CaMV35S promoter in transgenic crops and the corresponding detection methods were analyzed and summarized. Finally, aiming at the problems existing in detection of CaMV35S promoter in transgenic crops, it was proposed that in future studies, sequence information and detection data of related transgenic crops should be communicated and shared, meanwhile, data sharing and joint verification should be carried out among various testing institutions or laboratories, so as to establish a relatively uniform standard detection method for CaMV35S promoter.
Key words: CaMV35S promoter, transgenic crop, enhancer, PCR
中图分类号:
Q943.2
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