牛奶子花粉形态特征与生活力测定
摘要： 采用两种扫描电镜样品制备技术制备牛奶子花粉，扫描电镜观察其形态，研究结果表明：经自然干燥法制样，牛奶子花粉外观变形严重；采用固定—脱水制样，花粉外形保持良好，牛奶子花粉近球形，极面观钝三角圆形，具3沟，表面具脑纹状雕纹。采用I2-KI染色法测定牛奶子花粉开花后不同时间的生活力，结果表明：牛奶子花粉生活力在开花初较高，并在开花后36 h内维持在80%以上，之后迅速下降，但在花期末仍有部分花粉具有生活力。
关键词: 牛奶子, 花粉形态, 花粉生活力, 扫描电镜
Abstract: Different methods were applied to specimen preparation of Elaeagnus umbellata pollens for SEM observation. Results indicated that the natural drying pollen morphology of E.umbellata changed seriously, but pollen shape maintained well by the fixation and dehydration. The pollen grains of E.umbellata are subspheroid in equatorial view, triangle-boll and 3-colporate in polar view, and the exine sculpture is cerebroid. Viability of the pollen was measured by I2-KI staining in different time after anthesis. Results showed that the mean pollen viability is high in inchoate anthesis, and declines after sharply 36 hours, but a few pollen grains are still viable at the end of anthesis.
Key words: Elaeagnus umbellate, Pollen morphology, Pollen viability, SEM
中图分类号:
Q944.42
Q949.761.2
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