观赏芍药幼胚不定芽离体诱导研究
摘要： 通过对芍药幼胚离体培养，可以有效缩短芍药的育种周期。本文以‘粉玉奴’、‘朱砂判’、‘团叶红’三个芍药品种不同采收期的种子为材料，研究不同胚发育时期、不同外殖体类型、消毒方式及启动培养基配方对芍药不定芽诱导的影响。结果表明，‘朱砂判’和‘团叶红’较适合胚培养成苗的发育时期分别为花后50和70 d；‘朱砂判’的合子胚相比于带胚乳胚，更适于启动和成苗培养；外殖体消毒的最佳方法为将心皮用70%酒精处理30~60 s，再用2%NaClO消毒20 min，之后取出胚珠用2%NaClO处理10 min；适宜的启动培养基配方为1/2MS+1.0 mg·L-1 IAA+0.5 mg·L-1 GA3。
关键词: 芍药, 胚培养, 不定芽
Abstract: Culture of zygotic embryos is an effective way to accelerate the breeding process of herbaceous peony. With the zygotic embryos of three herbaceous peony(Paeonia lactiflora Pall.) cultivars(‘Fen Yu Nu’, ‘Zhu Sha Pan’ and ‘Tuan Ye Hong’), we studied the affection of different embryo age, explant types, sterilization methods and starting medium formula on root initiation in vitro. The suitable development period were 50 and 70 d after flowering for embryo-culture of ‘Zhu Sha Pan’ and ‘Tuan Ye Hong’, respectively. We got better results when using excised zygotic embryos rather than keeping part of endosperm reserved. The optimal disinfection method for explant was that firstly treating carpel for 30-60 s with 70% alcohol, then for 20 min with 2% NaClO, and finally for 10 min with 2% NaClO. The optimal initial medium was 1/2MS+1.0 mg·L-1 IAA+0.5 mg·L-1 GA3.
Key words: Herbaceous peony, embryo-culture, root initiation
相关知识
大花朱顶红鳞茎不定芽的诱导
裂叶秋海棠的离体快速繁殖
植物激素直接诱导非洲菊花蕾不定芽的研究
植物离体无性繁殖(包含脱毒).pptx
葱属植物离体培养研究进展
花烛离体培养与植株再生的优化研究
近十年秋水仙素离体诱导植物多倍体的研究进展,农艺学论文
百合花器官离体诱导小鳞茎的培养研究
朱顶红幼嫩花器官离体诱导试管小鳞茎的研究
几种金花茶离体诱导多倍体研究
网址: 观赏芍药幼胚不定芽离体诱导研究 https://m.huajiangbk.com/newsview1414442.html
| 上一篇: 芍药这样种，爆盆很简单，好看又漂 |
下一篇: 观赏芍药的花子能种植出来吗 |
