柠檬醛猴樟茎段组织培养技术研究
摘要： 柠檬醛猴樟是重要的园林绿化、用材、油用树种。以柠檬醛猴樟一年生枝条为材料，研究采集季节、消毒时间、激素对柠檬醛猴樟茎段组织培养的影响。结果表明：5月中旬，柠檬醛猴樟半木质化茎段组织培养用浓度为0.1% HgCl2消毒5 min，茎段腋芽萌芽率最高，褐化率和污染率较低。猴樟茎段萌芽适宜培养基为MS+1.0 mg·L-1 6-BA+0.05 mg·L-1 IBA，萌芽率为60%。增殖适宜培养基为MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA，增殖系数为4.33、株高为3.67 cm、地径为1.04 mm。生根培养11 d基部出现不定根，生根适宜培养基为MS+1.5 mg·L-1 IBA，生根率75.00%、根长5.06 cm、根数3.50、根粗0.82 mm。根数大于3的组培苗，移栽成活率80%以上。
关键词: 柠檬醛猴樟, 茎段, 组织培养
Abstract: Cinnamomum bodinieri var. citralifera is an important tree species for landscaping, timber and oil. We studied the effects of collection season, disinfection time and hormones on tissue culture of annual branches of C.bodinieri var. citralifera. The axillary bud germination rate of stem segments from semi-lignified stem segments of C.bodinieri var. citralifera was highest with 0.1% HgCl2 disinfected for 5 min. in the Mid-May, and the browning rate and pollution rate were low. The suitable medium for stem segment germination was MS+1.0 mg·L-1 6-BA+0.05 mg·L-1 IBA, with germination ratio 60%. The suitable medium for multiplication was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA, with multiplication coefficient of 4.33, plant height of 3.67 cm and ground diameter of 1.04 mm. Adventitious roots appeared at 11 d after culture of the base of tissue culture seedlings. The suitable medium for rooting was MS+1.5 mg·L-1 IBA. The rooting rate was 75.00%, the length of roots was 5.06 cm, the number of roots was 3.50, and the diameter of roots was 0.82 mm. Tissue culture seedlings with roots more than 3 had a survival rate of more than 80%.
Key words: Cinnamomum bodinieri var. citralifera, stem segment, tissue culture
中图分类号:
S792.23
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