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生防菌Y26的鉴定、发酵优化及其杀线虫活性测定

花匠小妙招
2025-01-05 14:51

摘要： 为防治黄瓜根结线虫病，本实验室前期从黄瓜根际土壤分离出的一株细菌Y26，测定其发酵液对根结线虫的生物活性，经发酵液处理后的根结线虫校正死亡率为95.12%，卵孵化的相对抑制率为83.51%，故菌株Y26可作为根结线虫生防菌资源；通过形态学观察及分子生物学方法鉴定菌株Y26为短小芽胞杆菌Bacillus pumilus；通过Plackett-Burma法和响应面分析试验得到菌株Y26发酵优化的条件是：玉米粉2.14%，豆饼粉3%，K2HPO4 0.57%，MgSO4·7H2O 0.21%，种龄12 h，pH为7.2，接种量2%，培养温度28℃，装瓶量为50 mL/250 mL，发酵优化后，菌液中芽胞浓度提高25.69%，10%发酵液对根结线虫的校正死亡率从优化前的78.03%提高到93.32%。研究结果表明，经发酵优化后的Y26菌液杀线虫活性显著提高，可作为杀线生物菌剂开发应用。

关键词: 根结线虫, 短小芽胞杆菌, 鉴定, 发酵条件优化

Abstract: To control the root-knot nematode disease in cucumber,a bacterial strain Y26 was isolated from cucumber rhizosphere soil in the laboratory.Bio-activity of fermentation broth against root-knot nematodes was determined.The corrected mortality rate of the second-stage juveniles treated with fermentation broth for 24 hours was 95.12%,and the relative inhibition rate of eggs hatching reached 83.51% after 48 hours of fermentation broth treatment,so strain Y26 can be used as a bacterial control resource for root-knot nematodes disease.Strain Y26 was identified as Bacillus pumilus by morphological observation and molecular identification.The optimized conditions for fermentation of strain Y26 by Plackett-Burma design and response surface analysis were corn flour 2.14%,bean cake flour 3%,K2HPO4 0.57%,MgSO4·7H2O 0.21%,cultural time 12 hours,pH 7.2,inoculation amount 2%,cultural temperature 28℃,and the media amount was 50 mL/250 mL.After fermentation optimization,the spore concentration was increased by 25.69%,the corrected mortality rate of 10% fermentation broth increased from 78.03% to 93.32% before optimization.The results showed that the activity of optimized Y26 fermentation broth root-knot nematodes was significantly increased and could be developed and applicated as a biological nematicide.

Key words: root-knot nematode, Bacillus pumilus, identification, optimization of fermentation conditions

中图分类号:

S476

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所属分类：花卉