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十字花科植物C2H2型锌指蛋白新基因BcMF20同源序列克隆与进化分析

花匠小妙招
2024-09-17 19:34

摘要

为获取C2H2型锌指蛋白基因在物种间的分类和进化关系,分析其在基因结构上的保守性,更好地研究该基因在植物花粉发育中的功能,根据白菜花粉发育相关基因 BcMF20 DNA序列设计引物,运用PCR技术分别从十字花科芸薹属和萝卜属18种材料中克隆了C2H2型锌指蛋白基因 BcMF20 的同源序列。经序列比对分析, BcMF20 同源基因在 DNA 序列上的相似性为86.9%~ 100%,所推导的氨基酸序列相似性为77.6%~100%,在锌指的保守区域,氨基酸序列完全相同。这些结果表明该基因具有较好的保守性,可能在十字花科植物的花粉发育中行使重要的功能。

Abstract

In order to elucidate categorization evolvement of C2H2 zinc finger protein, and clarify mechanism on C2H2 zinc finger protein in pollen development among plants at molecular level, the genes encoding C2H2 zinc finger protein analogues from 18 species of genera Brassica and Raphanus in Cuciferae were obtained by PCR strategy using specific primers designed from the full length of BcMF20 , a putative gene encoding C2H2 zinc finger protein which was related to the male sterility. The phylogenetic relationships of these species belonging to the family Cruciferae were investigated through comparison of the sequences. Homologous sequences of BcMF20 comparison indicated that the similarities among the genes at nucleotide and amino acid levels were 86.9% ~ 100% and 77.6%~100%, respectively. In the zinc finger regions of homologous sequences, the amino acid sequences were identical. These results showed that the BcMF20 was relative conservation in evolution in Cruciferae, and BcMF20 may play an important role in pollen development.

关键词

十字花科 /C2H2锌指蛋白 /BcMF20 /克隆 /系统发育{{custom_keyword}} /

Key words

Cruciferae /C2H2 zinc finger protein /BcMF20 /isolated /phylogenic development{{custom_keyword}} /

韩莹琰, 张爱红, 范双喜, 曹家树.十字花科植物C2H2型锌指蛋白新基因BcMF20同源序列克隆与进化分析[J]. 核农学报, 2011, 25(5): 916-921 https://doi.org/10.11869/hnxb.2011.05.0916

HAN Ying-yan, ZHANG Ai-hong, FAN Shuang-xi, CAO Jia-shu.CLONING AND EVOLUTIONARY ANALYSIS OF HOMOLOGOUS SEQUENCES OF A NOVEL GENE ENCODING C2H2 ZINC FINGER PROTEIN IN CRUCIFERAE[J]. Journal of Nuclear Agricultural Sciences, 2011, 25(5): 916-921 https://doi.org/10.11869/hnxb.2011.05.0916

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