茶树花提取物的抑菌和美白功效评价
摘要： 茶树花作为茶树的生殖生长产物，含有丰富的多酚、皂素、多糖、氨基酸和蛋白等多种活性物质，有抑制微生物生长和减少黑色素沉积的护肤作用。本文分析了茶树花及其提取物（Tea flower extract,TFE）的主要活性成分，并研究了其抑菌和美白功效的机理，为相关功效日化产品提供理论依据。研究的主要结果如下：茶树花及茶树花提取物的主成分种类相同，含量有一定差异，提取工艺后，茶树花提取物可溶性糖含量增加17.51%，多酚含量增加25%，氨基酸含量减少66%，皂素含量降低90%；TFE对金黄色葡萄球菌和大肠杆菌均具有显著的抑菌作用，最小抑菌浓度（MIC）分别为4.5 mg/mL和9.0 mg/mL；小鼠B16黑色素瘤细胞的体外美白实验结果表明，TFE可以通过改变细胞形态、抑制细胞增殖、抑制酪氨酸酶活性、减少黑色素生成等途径达到美白效果。
关键词: 茶树花提取物, 生化成分, B16色素瘤细胞, 抑菌, 美白
Abstract: As the reproductive growth product of tea tree (Camellia sinensis),tea flower is quite rich in bioactive compounds like polyphenols,saponins,polysaccharides,amino acids and proteins，which have antibacterial and whitening functions in some former researches.This study analyzes the bioactive compounds of tea flower and its extracts and evaluates its antibacterial and whitening effect,supporting further development of functional personal care products.The main experimental results are as follows:the main composition of the tea flower extract (TFE) and tea flower is the same,but the content is a little different;soluble carbohydrates increased by 17.51%,polyphenols increased by 25%,amino acids decreased by 66% and saponins decreased by 90% after being extracted.TFE had obvious antibacterial effect on Staphylococcus aureus and Escherichia coli.The minimum inhibitory concentrations (MIC) of tea flower and its extract were 4.5 mg/mL and 9.0 mg/mL respectively.TFE had whitening effect through changing cell morphology and inhibiting cell proliferation,tyrosinase activity and the melanin synthesis in the model of mice B16 melanoma cells in vitro.
Key words: tea flower extract, biochemical composition, B16 melanoma cells, antibacterial effect, whitening effect
中图分类号:
R285
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