金边瑞香醇提物化学成分的分离、鉴定以及抗氧化活性的研究的论文.doc 全文
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金边瑞香论文：金边瑞香醇提物化学成分的分离、鉴定以及抗氧化活性研究 【中文摘要】金边瑞香(Daphne odora var. marginata)系瑞香科(Thymelaeaceae)瑞香属(Daphne)落叶常绿灌木,为瑞香的变种。产自被誉为“中国瑞香之乡”江西省赣南大余县的金边瑞香是瑞香中的佳品,叶绿金黄色,花淡紫,花瓣先端5裂,白色,其基部紫红,香味浓烈,以“色、香、姿、韵”四绝著称于世,深受海内外花卉爱好者的亲睐,故有“牡丹花国色天香,瑞香花金边最良”之说。用叶治咽喉肿痛、齿痛、血疔热疖,用花治疗无名肿毒及各种皮肤病。本文对金边瑞香水醇相(70%乙醇)提取物褐色固体析出物的化学成分和抗氧化活性进行了研究,以期寻找新结构的化合物物或有生物活性的先导物,为金边瑞香的开发利用提供进一步的科学依据。第一章绪论部分,对金边瑞香植物化学成分和不同组分生物活性研究概况进行综述；概述了近年来天然产物中黄酮类化学成分以及金边瑞香中已知黄酮类化学成分的提取分离方法。第二章以金边瑞香醇提物所得褐色固体析出物为研究对象,采用硅胶、ODS、Sephadex LH-20柱色谱,分离得到十个单体化合物。第三章对金边瑞香中分离得到10个化合物的结构采用核磁共振波谱、质谱及紫外光谱等谱学数据进行分析,并与相关文献对照鉴定了他们的结构：荛花醇B (wikatrols B)、山奈酚(kaempferol)、瑞香黄烷B (daphnodorin B)瑞香黄烷D2 (daphnodorin D2)、瑞香黄烷A (daphnodorin A)、瑞香黄烷C(daphnodorin C)、瑞香黄烷D1 (daphnodorin D1)、瑞香黄烷I (daphnodorin I)、瑞香素(daphnetin),双白瑞香素(daphnoretin)。第四章拟采用清除DPPH自由基能力, 【英文摘要】Daphne odora var. marginata, family of Thymelaeaceae, is a famous flower and medical plant in the regions along the Yangzi River. It has been used in Chinese traditional medicine for many diseases. Their leaves have been mashed up to kurashiki the affected part for the ulcerate, injury, and mastitis; Their flowers soaked by white spirit have been used to cure rheumatoid arthritis, rheumatism, carbuncle, furuncle, miliaria, unknown toxin and traumatic injury. In this dissertation, the chemical constituents and antioxidant activity of the extracts from Daphne odora var. marginata were studied to find the main medicinal compounds with biological activity or novel structure. It would offer the theoretical foundation for the depth development and utilization of Daphne odora var. marginata.In the first chapter, we have described the progress of chemical constituents and pharmacological activity in Daphne odora var. marginata. Furthermore, the distribution and bioactivity of coumarin and daphnodorin which were characteristic components in Thymelaeaceae were reviewed.In the second chapter, the fractions was from Daphne odora var. marginata extracted by 70% ethanol, and then were subjected to column
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