全基因组关联分析揭示毛花猕猴桃果实品质和产量形成的候选基因
摘要：
果实的品质和产量是猕猴桃育种过程中首要关注的性状，但目前关于猕猴桃果实大小、形状和维生素C含量等性状的遗传机制研究非常有限，制约了猕猴桃分子育种的发展。本研究以毛花猕猴桃‘华特’自然杂交后代的140个个体为材料，通过全基因组重测序技术，共获得了888万个高度可信的SNP标记。对单果重、果实形状、维生素C含量、单枝花序数量等8个关键农艺性状进行了全基因组关联分析，定位了59个含有潜在功能基因的遗传位点，并在候选区间内鉴定到了与单果重、果实纵经、维生素C含量、单枝花序数量等性状相关的候选基因，如AeWUSCHEL, AeCDK1（细胞周期依赖激酶），AeAO1（抗坏血酸氧化酶），AeCO1（CONSTANS-like 4）等。通过构建AeAO1的RNAi载体，并注射到‘猕岛31’果实中干扰AeAO1基因的表达，结果表明‘猕岛31号’果实中抗坏血酸氧化酶活性显著下降，而维生素C含量显著增加。本研究通过全基因组关联分析的方法定位到了毛花猕猴桃果实品质和产量形成的候选基因，为猕猴桃分子标记辅助育种提供了新的见解。
Abstract:
Quality and yield are the primary concerns in kiwifruit breeding, but research on the genetic mechanisms of fruit size, shape, and ascorbic acid (ASA) content is currently very limited, which restricts the development of kiwifruit molecular breeding. In this study, we obtained a total of 8.88 million highly reliable single nucleotide polymorphism (SNP) markers from 140 individuals from the natural hybrid offspring of Actinidia eriantha cv. ‘White’ using whole genome resequencing technology. A genome-wide association study was conducted on eight key agronomic traits, including single fruit weight, fruit shape, ASA content, and the number of inflorescences per branch. A total of 59 genetic loci containing potential functional genes were located, and candidate genes related to single fruit weight, fruit length, ASA content, number of inflorescences per branch and other traits were identified within the candidate interval, such as AeWUSCHEL, AeCDK1 (cell cycle dependent kinase), AeAO1 (ascorbic oxidase) and AeCO1 (CONSTANS-like 4). After constructing an RNAi vector for AeAO1 and injecting it into the fruit of cv. ‘Midao 31’ to interfere with the expression of the AeAO1 gene, the results showed that the activity of ascorbic oxidase in the fruit of ‘Midao 31’ significantly decreased, while the content of ASA significantly increased. This study provides valuable insights into the genetic basis of variation in A. eriantha fruit traits, which may benefit molecular marker-assisted breeding efforts.
Key words: Actinidia eriantha , GWAS, SNP , QTLs , fruit quality
相关知识
全基因组关联分析确定毛花猕猴桃果实品质和产量的候选基因,Journal of Integrative Agriculture
野生毛花猕猴桃asa含量的关联分析
野生毛花猕猴桃种质资源内在品质遗传多样性及SSR关联分析
菊花耐涝性的全基因组关联分析及候选基因挖掘
科学网—再探猕猴桃基因奥秘
菊花主要园艺性状的关联分析及候选基因功能鉴定.ppt
再探猕猴桃基因奥秘—新闻—科学网
萱草属植物开花节律全基因组关联分析
我校在毛花猕猴桃雄性资源农艺性状相关基因研究方面取得新进展
综合代谢组和转录组分析揭示毛花猕猴桃可剥性形成的相关机制,Scientia Horticulturae
网址: 全基因组关联分析揭示毛花猕猴桃果实品质和产量形成的候选基因 https://m.huajiangbk.com/newsview2311526.html
| 上一篇: 半亩花田 |
下一篇: 基于花前物候利用灰色关联分析法建 |
