牡丹叶斑病病原真菌鉴定及生物学特性分析
牡丹叶斑病病原真菌鉴定及生物学特性分析-identification and biological characteristics analysis of pathogenic fungi of peony leaf spot
摘要 摘要 optimum growth pH was 6, sporulation was a maximum of pH 11. Light had no significant effect on mycelial growth and sporulation. Glucose and yeast were the most suitable carbon and nitrogen sources for mycelial growth. Conidia germinated during RH 65%-100% and the germination rate increased with the increase of RH. when RH is saturated, spore reached its maximum germination rate 94.51%. The lethiferous temperature of conidia was 51℃ for l0 min. In this study, based on morphology, pathogenic fungi of Peony leaf spot were further identified at the molecular level. The results indicate that Pilidium concavum and Discosia sp. are new pathogens on Peony in our country. Biological characteristics of P. concavum had a great significance on further revealing the prevention and treatment of fungal diseases of peony. KEY WORDS: Peony, leaf spot diseases, pathogenic fungi, morphological identification, molecular identification, biological characteristics Dissertation Type: Applied basic research IV 缩略语词汇表 缩略语词汇表 缩略语词汇表 Abbreviations 缩写词英文全名中文全名 PDAPotato dextrose agar马铃薯葡萄糖琼脂培养基 DNADeoxynculeoside acid脱氧核糖核苷酸 ITSInternal transcribed spacer内转录间隔区 GenBankGenBank美国 DNA 序列数据库 TrisTris(Hydroxymethyl)aminomethane三羟甲基氨基甲烷 EDTAEthylene Diamine Tetraacetic Acid乙二胺四乙酸 TETris EDTATE 缓冲液 TAETris Acetate EDTATAE 电泳缓冲液 EBEthidium bromide溴化乙锭 CTABHexadecyl trimethyl ammonium Bromide十六烷基三甲基溴化铵 PCRPolymerase Chain Reaction聚合酶链式反应 dNTPDeoxy-ribonucleoside triphosphate三磷酸脱氧核糖核苷 BLASTBasic Local Alignment Search Tool局部相似性查询工具 ODOptical Density光密度 DDBJ DNA Data Bank of Japan 日本 DNA 数据库 EMBLEuropean Molecular Biology Laboratory 欧洲 DNA 数据库 PDB Protein date bank 蛋白质数据库 NCBINational Center of Biotechnology Information美国国立生物技术信息中心 bpBase pair碱基对 PSAPotato sucrose agar马铃薯蔗糖琼脂培养基 CzapekCzapek’s medium查氏培养基 OMAOatmeal agar燕麦片琼脂培养基 CMACor
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