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苏芳花花色素体外抗氧化性及抑菌性研究

苏芳花花色素体外抗氧化性及抑菌性研究

The Antioxidant Activity and Antibacterial Activity of Pigment from Cercis chinensis in Vitro

DOI:10.3969/j.issn.1673-1689.2012.06.017

中文关键词: 苏芳花 花色素 抗氧化性 抑菌性

英文关键词: Cercis chinensis pigment antioxidantion antibacterial

基金项目:淮海工学院自然科学基金项目(Z2010036)

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中文摘要:

从苏芳花鲜花瓣提取分离花色素,研究其在体外的抗氧化性和抑菌性。结果表明:苏芳花花色素具有较强的还原性,能有效清除系统中的超氧阴离子和羟基自由基,且清除效果与色素浓度正相关。在苏芳花花色素浓度为0.48 mg/mL时,其还原力与0.1 mg/mL VC相当,而对超氧阴离子和羟基自由基的清除率则可分别达到59.3%和96.1%。苏芳花花色素能够显著抑制大肠杆菌、金黄色葡萄球、枯草芽孢杆菌的生长。在色素浓度为0.48 mg/mL时,对3种菌的抑菌圈直径分别为9.46 mm、9.36 mm和8.14mm。苏芳花花色素具有较强的抗氧化性和抑菌性。

英文摘要:

The objective of this manuscript is to measure and analysis the antioxidant and antibacterial activity of the pigment which is extracted from the flower of Cercis chinensis.A sequence of experiments was conducted to measure the above parameters,such as spectrophotometry and inhibiting bacteria circle diameter measure.The results demonstrate that the pigment has strong reduction and is able to effectively scavenge hydroxyl radical and superanion radical in vitro.Moreover,its scavenging effect has positive correlation with its concentration. When the pigment concentration is 0.48 mg/mL,the reducing power is identical to that of 0.1 mg/mL VC,and the hydroxyl radical and superanion radical eradication rates are 59.3%and 96.1%,respectively.At the same concentration,the pigment has significant concentrationdependent inhibitory effect against E.coli and Staphyloccus aureus and Bacillus subtilis.Diameters of inhibiting bacteria circle of the above three kinds of bacteria are 9.46 mm,9.36 mm, 8.14 mm,respectively.From the aboved results,it can concluded that the pigment has strong antioxidant and antibacterial activity.

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