基于转录组分析内源激素在调控枇杷花发育进程中的作用
摘要：
为研究枇杷(Eriobotrya japonica Lindl.‘Ninghaibai’)花发育机制,以花芽生理分化期(EjS1)、花芽形态分化期(EjS2)、花穗发育期(EjS3)、小花发育期(EjS4)和开花期(EjS5)这5个时期的当年生春梢上顶芽、花芽或花穗为试验材料进行转录组测序分析,筛选出相关性最高的代谢通路。测定花发育过程中生长素(IAA)、脱落酸(ABA)、赤霉素(GA)和玉米素核苷(ZR)等激素水平的变化,并对激素代谢与信号转导相关基因,以及花发育相关MADS-box家族基因的花发育时期特异性表达模式进行分析;同时,通过实时荧光定量PCR(qRT-PCR)对部分花发育相关基因进行验证。结果表明:不同发育时期差异表达基因在植物激素代谢和信号转导途径中显著富集;低含量IAA、GA与高含量ABA有利于枇杷花芽的形成,较高水平GA和ABA能促进枇杷开花;高水平ZR能促进枇杷花芽分化和花穗形成,并可能与花器官分化有关。植物激素代谢和信号转导途径相关基因相互作用调节激素水平变化,并与MADS-box家族基因共同调控枇杷花发育,研究结果有助于阐明内源激素在枇杷花发育进程中的作用机制。
关键词: 枇杷, 花发育, 转录组, 内源激素
Abstract:
To study the flower development mechanism in Eriobotrya japonica Lindl. ‘Ninghaibai’, transcriptome analysis was performed on the apical buds, floral buds, or inflorescences at 5 different stages, including physiological differentiation stage (EjS1), morphological differentiation stage (EjS2), inflorescence emergence and expansion stage (EjS3), single flower development stage (EjS4) and blossoming stage (EjS5). The aim was to identify the highest correlated metabolic pathways associated with the flower developoment. The changes of hormone levels of auxin (IAA), abscisic acid (ABA), gibberellin (GA), and zeatin riboside (ZR) during flower development were determined, and the specific expression patterns of hormone metabolism and signal transduction genes as well as MADS-box family genes were analyzed. Some flower development-related genes were verified by real-time quantitative PCR (qRT-PCR) analysis. The results showed that differentially expressed genes at different developmental stages were significantly enriched in plant hormone metabolism and signal transduction pathways. Low content of IAA and GA and high content of ABA were beneficial to the formation of loquat flower buds, while high level of GA and ABA promoted the flowering of loquat. High levels of ZR promoted flower bud differentiation and panicle formation in loquat, and may be related to flower organ formation. The genes related to plant hormone metabolism and signal transduction pathway interact with each other to regulate hormone levels, and together with MADS-box family genes to regulate the development process of loquat flowers. This study may contribute to clarifying the mechanism of endogenous hormones in the development process of loquat flowers.
Key words: loquat, flower development, transcriptome, endogenous hormone
中图分类号:
S667.3
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