低温胁迫对茶树抗氧化酶活性的影响
摘要： 以白鸡冠F1代新品系（0306I、0306F、0306D）及黄旦的扦插苗为试验材料,测定低温（0℃）胁迫下茶树抗氧化酶活性的变化。结果表明,低温下各品种（系）叶片电导率、过氧化氢及丙二醛含量都显著增加,其中0306F与0306D增加幅度明显大于黄旦与0306I;谷胱甘肽还原酶（GR）、抗坏血酸过氧化物酶（APX）、过氧化物酶（POD）与超氧化物岐化酶（SOD）活性都显著增加,其中黄旦与0306I低温下GR、APX、POD、SOD增加幅度远大于品系0306D与0306F。低温下单脱氢抗坏血酸还原酶（MDAR）、脱氢抗坏血酸还原酶（DHAR）、过氧化氢酶（CAT）、抗坏血酸（ASC）与谷胱甘肽（GSH）活性显著下降,其中0306F与0306D品系叶片的MDAR与CAT低温下下降幅度远大于黄旦与0306I。回归分析发现,叶片双氧水含量与APX（y=601.8-59.1x）及CAT（y=5.45-0.77x）活性呈负相关,电导率与ASC（y=7.45-10.35x）也呈负线性相关。以上结果表明,茶树品种（系）0306F与0306D抗寒性弱于0306I与黄旦。
关键词: 低温, 茶树, 活性氧, 代谢
Abstract: New clonal tea varieties 0306D, 0306F, 0306I from F1 population of Baijiguan and Huangdan were employed as test materials. Antioxidant enzyme activities of tea plants under low temperature were measured. The results showed that leaves under low temperature had higher conductivity, malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents with the increasing ratios higher in 0306D and 0306F than 0306I and Huangdan. The increasing ratios of peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) activities of the Huangdan and 0306I tea varieties were higher than 0306D and 0306F. While monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), ascorbate (ASC), reduced glutathione (GSH) and catalase (CAT) activities of 0306D and 0306F were less decreased than 0306I and Huangdan. The results indicated that higher activities of defense enzymes and higher antioxidant content in 0306I and Huangdan under stress were associated with chilling tolerance. Further regression analysis showed that the H2O2 content was negatively correlated with APX and CAT, and conductivity was negatively correlated with ASC content. The above results indicated that the tolerance to low temperature in 0306I and Huangdan was stronger than 0306F and 0306D.
Key words: low temperature, tea plant, active oxygen, metabolism
中图分类号:
S571.1
S233.75
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