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灰霉菌Atg1基因的致病功能研究.pdf 文档全文预览

花匠小妙招
2024-11-26 15:55

中文摘要灰霉菌（Botrytis cinerea ）是一种能够引起植物灰霉病的病原真菌，其能够侵染超过 1400 种的植物，并且在植物的各个发育时期均能致病，对于其的防治研究迫在眉睫。据报道，灰霉菌在入侵植物的时候会激发自身的一种名为自噬的机制帮助其顺利入侵植物，自噬相关基因Atg1 （Autophagy-related gene 1 ）在哺乳动物和细菌中的作用已研究的非常清楚，而在灰霉菌中还少见报道，其对灰霉菌致病力是否有影响值得深入研究。因而本实验主要目的为探究灰霉菌 Atg1 基因对于灰霉菌致病力是否有影响，主要研究内容为：1.构建灰霉菌 Atg1 基因敲除载体。2.利用所构建载体进行灰霉菌 Atg1 基因敲除，获得Atg1 基因敲除突变体。 3.初步探究 Atg1 基因敲除突变体相比于野生型致病力的变化情况。通过本实验成功构建了Atg1 基因敲除载体，利用敲除载体成功获得了Atg1 基因敲除突变体，通过接种实验对Atg1 基因敲除突变体与野生型的致病力进行比较，发现 Atg1 基因敲除突变体致病力明显下降。关键词：灰霉菌；自噬；Atg1 基因；基因敲除 I Abstract Botrytis cinerea is a pathogenic fungus that can cause Botrytis cinerea, which can infect more than 1,400 species of plants and can be pathogenic in the various developmental stages of the plant,so the research on the control of Botrytis cinerea is imminent. Reported that Botrytis cinerea in the invasion of plants will active a mechanism called autophagy to help its invasion.The effect of autophagy-related gene Atg1 has been studied very clearly in mammals and bacteria, but it is rarely reported in gray mold,and its impact on the pathogenicity of Botrytis cinerea that is worthy of further study. Therefore, the main purpose of this study is to investigate whether the Atg1 gene of Botrytis cinerea has an effect on the pathogenicity of Botrytis cinerea . The main contents are as follows: 1. Construction of Atg1 gene knockout vector. 2. Obtaining the Atg1 knockout mutant by using the constructed knockout vector. 3. To explore the changes of pathogenicity of Atg1 gene knockout mutations compared with wild type. Through the experiment,we successfully constructed the Atg1 gene knockout vector, and successfully obtained the Atg1 knockout mutant by knockout vector. Through inoculation experiments, we investigated t

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