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胶体金免疫层析产品快速检测农药残留的评价与分析

花匠小妙招
2024-11-30 20:40

摘要: 本文建立一种快速检测试剂的质量评价方法。采用空白基质加标方式制备盲样，并验证盲样的均匀性；每个快检产品随意抽取3个不同批次，每批次产品均检测50份盲样，其中阴性样品、阳性样品至少20份。对市售的14个农药残留快速检测产品的特异性、准确性（假阳性率、假阴性率）等参数进行了评价，共完成了2100份盲样检测。结果表明，14个产品中，11个产品通过评价，通过率为78.6%，1个产品假阳性率较高，2个产品的假阴性率较高；全部产品的特异性均较好。本方法可对快速检测产品进行科学客观的质量评价，进而筛选出质量稳定可靠的快速检测产品，为基层监管部门在果蔬产品监管方面提供技术支持。

Abstract: In this work,a quality evaluation method for rapid detection kits was established. The blind samples were prepared by spiking blank matrices and the homogeneity and the stability of them were verified simultaneously. Three different batches of rapid detection kits were randomly selected from each product,and each one was used to detect 50 blind samples which contained at least 20 positive and 20 negative samples. The specificity and accuracy(false positive and false negative)and other parameters of 14 commercially available assay kits for the rapid screening of pesticide residue were evaluated with a total of 2100 blind samples. The results showed that,11 of 14 of rapid detection kits passed the evaluation with a pass rate of 78.6%. One product had a higher false positive rate and two had a higher false negative rate;all the products had good specificity.This method could scientifically and objectively evaluate the quality of rapid testing products,and then screen out the rapid testing products with stable and reliable quality,and would provide technical support for grassroots regulatory authorities in the supervision of fruit and vegetable products.

[1] 徐秋生,贺慧琳,朱吕,等. 高效液相色谱法测定番茄中腐霉利和异菌脲的残留量[J]. 食品安全质量检测学报,2017,8(2):486-490. [2] 杨莉,冯光泉,张文斌,等. 固相萃取-GC-ECD法分析三七中腐霉利的消解动态及残留规律[J]. 农药,2018,57(12):908-911. [3]

Watanabe Eiki,Kobara Yuso,Baba Koji,et al. Aqueous acetonitrile extraction for pesticide residue analysis in agricultural products with HPLC-DAD.[J]. Food chemistry,2014,154:7-12.

[4]

Chao Feng,Qian Xu,Xinlei Qiu,et al. Comprehensive strategy for analysis of pesticide multi-residues in food by GC-MS/MS and UPLC-Q-Orbitrap[J]. Food Chemistry,2020,320:126576.

[5]

Li Y,Kim M R,Lee K B,et al. Determination of procymidone residues in ginseng by GC-ECD and GC-MS equipped with a solvent-free solid injector[J]. Food Control,2007,18(4):0-368.

[6]

Chen L,Yin L,Song F,et al. Determination of pesticide residues in ginseng by dispersive liquid-liquid microextraction and ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chromatography B,2013,918-918:71-77.

[7]

Ferrer CM,Jose Gómez,Juan F. García-Reyes,et al. Determination of pesticide residues in olives and olive oil by matrix solid-phase dispersion followed by gas chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry[J]. journal of chromatography A,2005,1069(2):183-194.

[8] Shi C,Zhao S,Zhang K,et al. Preparation of colloidal gold immunochromatography strip for detection of methamidophos residue[J]. 环境科学学报:英文版,2008,(11):1392-1397. [9] 全国人民代表大会常务委员会. 中华人民共和国食品安全法[EB/OL].[2018-12-29]. http://search.chinalaw.gov.cn/search2.html [10] 杨菊芬. 快速检测技术在食品安全监管中的应用[J]. 现代食品,2016,(24):31-32. [11]

Jiang J,Zhao S L,Huang Y,et al. Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection[J]. Journal of Chromatography A,2013,(1282):161-166.

[12]

Hendrickson Olga D,Zvereva Elena A,Zherdev Anatoly V,et al. Development of a double immunochromatographic test system for simultaneous determination of lincomycin and tylosin antibiotics in foodstuffs[J]. Food Chemistry,2020,318:126510.

[13]

Li Xiangmei,Wu Xinze,Wang Jin,et al. Three lateral flow immunochromatographic assays based on different nanoparticle probes for on-site detection of tylosin and tilmicosin in milk and pork[J]. Sensors and Actuators B:Chemical,2019,301:127059.

[14]

Hua X,Qian G,Yang J,et al. Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples[J]. Biosensors & bioelectronics,2010,26(1):189-94.

[15] 谢刚,叶金,王松雪. 食品安全快速检测方法评价技术研究进展[J]. 食品科学,2016,37(17):270-274. [16] 王硕. 食品安全快速检测技术研究动态[J]. 食品安全质量检测学报,2014,(7):1911-1912. [17] 黄怡淳,丁炜炜,张卓旻,等. 食品安全分析样品前处理-快速检测联用方法研究进展[J]. 色谱,2013,31(7):613-619. [18] 郑天驰,王钢力,曹进,等. 食品快速检测方法现状及建议[J]. 食品安全质量检测学报,2016,7(3):853-859. [19] 国家质量监督检验检疫总局. SN/T 2775-2011商品化食品检测试剂盒评价方法[S]. 北京:中国标准出版社,2011. [20] 申雷,刘祥,史长生,等2种快速检测产品6类理化项目的质量评价[J]. 食品安全质量检测学报,2019,10(14):4636-4644. [21] 罗俊霞,赵建波,刘伟,等. 农产品质量安全快速检测试剂盒评价方法研究[J]. 农产品质量与安全,2019,(2):62-66. [22] 李涛,孙宁宁,刘海静,等. 水产品中氯霉素快速检测产品的验证性评价及分析[J]. 食品安全质量检测学报,2018,9(11):2596-2601. [23] 李菊,谢建军,王璐,等. 基于质量评价的孔雀石绿快检试剂盒筛选策略[J]. 食品安全质量检测学报,2020,11(3):875-882. [24] 刘欢,郭萌萌,李晋成,等. 免疫胶体金快速检测水产品中孔雀石绿残留的质量分析和评价[J]. 食品安全质量检测学报,2015,6(5):1675-1682. [25] 史海莹,姜洁,翟洪杰,等. 吊白块快检试剂盒分析与评价[J]. 食品科技,2012,37(8):290-292.