无患子皂苷的提取工艺及生物活性研究
为更好地利用无患子，研究无患子皂苷的提取工艺及生物活性。通过无患子果皮粉粹、溶液抽滤、旋转蒸发等过程获取无患子皂苷粗提物；针对粗提物，利用正丁醇等试剂，通过溶解、分层萃取与旋转蒸发等过程获取无患子皂苷精产物；选取齐墩果酸为标准品绘制标准曲线，计算皂苷含量、得率与纯度。利用光电比色计确定皂苷去污力，通过试管二倍稀释法检测抑菌活性，基于羟自由基作用测定方法测试抗氧化性。实验结果显示：原料粉粹处理且粒度为20~60目条件下，无患子皂苷纯度与产率更高；无水乙醇溶液浓度为70%时，皂苷得率达到上限；萃取比例为1:12时皂苷含量最高；发酵后皂苷液去污性能与抑菌性能高于皂苷浓缩液；无患子皂苷抗氧化活性较好。
服务把本文推荐给朋友加入引用管理器 E-mail AlertRSS（）作者相关文章许应生关键词: 无患子 皂苷 提取工艺 生物活性 去污力 抑菌性 Abstract:The extraction process and biological activity of soapberry saponin were studied in order to make better use of soapberry. The crude extract of soapberry saponin was obtained by the process of soapberry peel powder, solution suction filtration, rotary evaporation and other processes. N-butanol and other reagents were used to obtain soapberry saponin essence products through dissolution, layered extraction and rotational evaporation for crude extracts. Oleanolic acid was selected as the standard to draw a standard curve, and the saponin content, yield and purity were calculated. The saponin detergency detergency was determined by photoelectric colorimetry, the antibacterial activity was detected by the twofold dilution method of the test tube, and the antioxidant activity was tested based on the determination method of hydroxyl radical action. The experimental results showed that the purity and yield of soapberry saponin are higher under the condition of raw material powder treatment and particle size of 20-60 mesh. When the concentration of absolute ethanol solution is 70%, the yield of saponin reaches the upper limit. When the extraction ratio is 1:12, the saponin content is the highest. The decontamination and antibacterial properties of saponin solution are higher than those of saponin concentrate after fermentation. The antioxidant activity of soapberry saponins is good.
Key words: Soapberry Saponin extraction technology biological activity cleaning ability bacteriostasis 出版日期: 2023-07-25 发布日期: 2023-07-25 整期出版日期: 2023-07-25[1]. [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 0.[2]. [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 0.[3]SHAO Bao-li, LU Da, ZHAO Dong-hui.The Application of Dimensional Analysis in the Physical Quantity Conversion between Physical System and Numerical System [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 1-3.[4]ZHANG Jian, ZHAO Xiang, QU Bo, WU Qi, LIU Yu-tong, LI Yu-shi, LIU Qun.Application of Phosphorus-sulfur-nitrogen Composite Flame Retardant in Cotton Fabric [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 4-7.[5]WU Ping, REN Hong, LU Fei, WEI Qingling.A Functional Material on Recognition of Zn(II) ions based on the New Azo Compound [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 8-10.[6]YANG Yan-jun, WANG Ya-hong, Yang Xiu-dong.Process Aptimization of Surfactant Assisted Extraction of Total Polyphenols from Kyllinga Brevifolia Rottb [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 11-15.[7]LIU Jin-lu, LEI Yong-ping, WANG xiao-lin, ZHONG fang-li.Study on the Purification Method of Total Saponins fromFruit of Rosa Davuvrica Pall. and its Purification Method [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 16-23.[8]SONG Jian-gang, ZHONG Fang-li, WANG Xiao-lin, LIN Yu.Study on Extraction of Anthocyanin from Aronia melanocarpa Fruit by Ionic liquid Ultrasound Assisted [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 24-31.[9]TAN Li-hui, TAN Hong-wu.The Crashworthiness Analysis of different Cross-Section Thin-Walled Components [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 32-35.[10]YU Wen-xin, ZHENG Kai, WANG Li-hui, LIU Hai-bo, Wang Jian-xin.The Influence of Magnetostrictive Transducer Radiation Plate material on Radiation Sound Field Distribution [J]. Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 36-40.ViewedFull textAbstract
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