Suitable Cryo
作 者 ：陈丹;赵洁
期 刊 ：武汉植物学研究 2005年 23卷 3期 页码：285-290
关键词：花器官；冰冻切片技术；
Keywords:Floral organs, Cryo-sectioning technique,
摘 要 ：通过对4种植物主要花器官冰冻切片技术的各个环节及参数的研究,建立了一种适合于植物花器官的冰冻切片技术,即蔗糖保护-液氮速冻-冰冻切片法。其具体程序是:材料经固定和冷冻保护(蔗糖为冷冻保护剂)后进行速冻包埋(液氮为包埋剂);尔后进行冰冻切片;切片经干燥和染色(或者不染色)后,在显微镜下观察并摄影。此法为植物花器官的细胞生物学和分子生物学研究提供了简便、快速和高效的切片技术。
Abstract:With studying on the technical parameters of cryo-sectioning in floral organs of four plant species, we established a suitable cryo-sectioning method for floral organs of plants. The procedure consisted of several steps as follows.The materials were taken out and fixed in solution contained cryo-protected reagent (sucrose). The fixed samples were embedded, frozen in liquid nitrogen and cryo-sectioned. Then, the sections were dried, stained or not and photographed. This method was better than single liquid freezing or sucrose-protecting or direct cryo-section. The fine structures of ovaries, anthers and shoot apical buds in four plant species were displayed by the sucrose-protecting and liquid nitrogen frozen cryo-sectioning technique. One important point is that the different concentrations of sucrose were used during fixing because of varied plant tissue structures and osmotic pressure, and the sucrose level suitable for the floral organs of the four species were also investigated.
鄂ICP备06018747号 Copyright @ 2017
植物通 All Rights Reserved
Email：23198511@qq.com
回顶部
相关知识
The suitable distribution of alpine plant Sorbus tianschanica and its environmental driving factors
Effect of future climate change on suitable areas of Rhododendrons in Guangdong
气候变化情景下大花杓兰在中国的适生区预测
气候变化对灯盏花(Erigeron breviscapus)在中国潜在适生区的影响
气候变化下濒危植物半日花在中国的潜在分布
软枣猕猴桃在中国的适生区分析及对未来气候变化的响应
气候变化对黄山花楸潜在地理分布的影响
基于Maxent模型的互花米草潜在生境适宜性分析
未来气候变化对粤港澳地区杜鹃花适生区的影响
光照强度、温度、pH、盐度对小球藻（Chlorella）光合作用的影响
网址: Suitable Cryo https://m.huajiangbk.com/newsview1155717.html
| 上一篇: 植物石蜡切片技术实验简介、原理、 |
下一篇: 植物细胞结构与植物徒手切片观察试 |
