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毛竹茎秆纤维发育过程中Ca 2+—ATPase分布的动态变化

花匠小妙招
2024-12-31 00:55

竹茎秆快速生长期淀粉分解相关酶基因表达的分析栗青丽，王灵杰，高培军，韦赛君，吕嘉欣，高岩，张汝民（浙江农林大学省部共建亚热带森林培育国家重点实验室，浙江杭州 311300）摘要：【目的】揭示毛竹Phyllostachys edulis 茎秆内α-淀粉酶(AMY)和β-淀粉酶(BAM)以及相关基因表达对毛竹快速生长的调控机制。

【方法】以毛竹笋竹为试材，测定茎秆不同时间(10:00、14:00、18:00、22:00、2:00、6:00)和不同节间(第7、10、13、16节)内非结构性碳水化合物(NSC)质量分数、α-淀粉酶活性和β-淀粉酶活性以及PeAMY 和PeBAM 基因表达。

【结果】毛竹茎秆NSC 质量分数白天较高，18:00之后逐渐降低，第7节和第10节葡萄糖质量分数2:00分别比18:00降低了25.4%和27.2%(P ＜0.01)，果糖分别降低了50.0%和34.1%(P ＜0.01)，蔗糖分别降低了49.8%和27.4%(P ＜0.01)，淀粉6:00分别比18:00降低了27.3%和23.2%(P ＜0.01)；BAM 活性和基因表达在白天较稳定，18:00之后极显著升高，黎明前逐渐下降，第7、10和13节22:00活性分别比18:00高90.5%，76.7%和50.5%(P ＜0.01)，PeBAM 基因表达2:00分别比18:00高1.8、1.8和1.7倍(P ＜0.01)。

【结论】毛竹茎秆快速生长期白天生长慢，夜间生长快，茎秆发育和成熟是从下往上顺次推进的。

毛竹茎秆快速生长与PeBAM 基因的表达密切相关，BAM 在毛竹茎秆淀粉降解中可能起主要作用。

图4表1参38关键词：林木育种学；毛竹；快速生长；非结构性碳水化合物；淀粉酶；基因表达中图分类号：S722.3 文献标志码：A 文章编号：2095-0756(2020)06-1128-08Gene expression of starch decomposing enzymes in Phyllostachys edulisstems during the rapid growth periodLI Qingli ，WANG Lingjie ，GAO Peijun ，WEI Saijun ，LÜ Jiaxin ，GAO Yan ，ZHANG Rumin（State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China ）Abstract: [Objective ] The aim of this study is to reveal the regulating mechanism alpha amylase (AMY) and beta amylase (BAM) and related gene expression of Phyllostachys edulis in the rapid growth period. [Method ]The stems of Ph. edulis shoots were used as materials, and the content of non-structural carbohydrates (NSC),AMY and BAM activity, and gene expression of PeAMY and PeBAM were measured at different time (10:00,14:00, 18:00, 22:00, 2:00, 6:00) and different internodes (7, 10, 13, 16). [Result ] The NSC content of bamboo stems was higher in the daytime, and gradually decreased after 18:00. At 2:00, the glucose content in the 7th and 10th internodes decreased by 25.4% and 27.2% (P ＜0.01), fructose by 50.0% and 34.1%, respectively (P ＜0.01), sucrose by 49.8% and 27.4%, respectively (P ＜0.01). Starch content at 6:00 decreased by 27.3% and 23.2%, respectively (P ＜0.01). The activity and gene expression of BAM were stable in the daytime, increased significantly after 18:00, and gradually decreased before dawn. BAM activity of the 7th, 10th and 13th internodes was 90.5%, 76.7% and 50.5% higher at 22:00 than at 18:00 (P ＜0.01). The PeBAM gene expression was 1.8, 1.8 and 1.7 times higher at 2:00 than at 18:00 (P ＜0.01). [Conclusion ] During the period of rapid growth, the stem of Ph. edulis grows slowly in the daytime and quickly at night. The development and收稿日期：2019-11-05；修回日期：2020-05-24基金项目：国家自然科学基金资助项目(31570686)作者简介：栗青丽，从事植物生理生态学研究。