猕猴桃花粉活力测定方法研究
摘要： 以中华猕猴桃（Actinidia chinensis）为样本,比较了制粉方法对花粉活力的影响,采用TTC法、I2-IK法和离体萌发法测定花粉活力,并用“3414”试验设计对蔗糖、硼酸、氯化钙浓度进行筛选,建立测定猕猴桃花粉活性的合适方法。结果表明,将花粉与花粉壳混合后更有利于花粉管的萌发;氯化三苯基四氮唑（TTC）和碘-碘化钾（I2-IK）不适用于猕猴桃花粉活力的测定,离体萌发法能有效地反映花粉的真实活力;离体萌发法的最佳配比为10%蔗糖+0.1 mg/mL硼酸+0.1 mg/mL氯化钙,萌发率可达77.9%。
关键词: 猕猴桃（Actinidia chinensis）, 花粉活力, 氯化三苯基四氮唑, 碘-碘化钾, 离体萌发法
Abstract: Taking Actinidia chinensis as sample, this article compared the effects of pollen preparation methods on pollen vitality. The pollen vitality was studied using TTC method, I2-IK method and an in vitro method, and the optimical concerntration of sucrose, H3BO3 and CaCl2 were selected by ‘3414’ test to find a proper method for measuring kiwifruit pollen vitality. The result show that mixing pollen with pollen shells is more conducive to germination of pollen tubes. TTC and I2-IK are not suitable for the determination of kiwi pollen activity. The in vitro method can effectively reflect the true vitality of pollen. The optimal medium was 10% sucrose+0.1 mg/mL H3BO3+0.1 mg/mL CaCl2 on pollen germination in vitro and the pollen germination ratio was up to77.9%.
Key words: kiwifruit（Actinidia chinensis）, pollen vitality, TTC, I2-IK, in vitro germination
中图分类号:
S663.4
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