霸王花提取物抗氧化和抗菌活性研究
LI Ben-jie, HUANG Mao-chun, LIAO Yan-fang, CHEN Rui, LV Jin-yan. Antibacterial and antioxidant activity of different extracts from flowers of Hylocereus undatus[J]. Science and Technology of Food Industry, 2014, (07): 80-82. DOI: 10.13386/j.issn1002-0306.2014.07.017
Citation: LI Ben-jie, HUANG Mao-chun, LIAO Yan-fang, CHEN Rui, LV Jin-yan. Antibacterial and antioxidant activity of different extracts from flowers of Hylocereus undatus[J]. Science and Technology of Food Industry, 2014, (07): 80-82. DOI: 10.13386/j.issn1002-0306.2014.07.017
霸王花提取物抗氧化和抗菌活性研究
1. 广西中医药大学赛恩斯新医药学院
2. 广西化工研究院
详细信息
作者简介:李本杰 (1984-) , 男, 本科, 助理实验师, 主要从事中药有效成分分离提取和活性方向的研究。;
中图分类号: TQ914
计量 文章访问数: 0142 HTML全文浏览量: 018 PDF下载量: 0278Antibacterial and antioxidant activity of different extracts from flowers of Hylocereus undatus
Faculty of Chinese Medical Science Guangxi University of Chinese Medicine
Guangxi Research Institute of Chemical Industry
摘要
摘要: 目的:研究霸王花不同溶剂提取物的体外抗氧化和抗菌活性。方法:用冷浸法得到霸王花乙醇提取物(EE)、丙酮提取物(AE)和乙酸乙酯提取物(EAE),通过测定三种提取物的ABTS自由基清除能力、DPPH自由基清除能力以及还原能力来评估其抗氧化能力;通过纸片扩散法和试管法评估霸王花三种提取物的抗菌活性。结果:霸王花不同溶剂提取物均表现出抗氧化性能,其中乙酸乙酯提取物(EAE)具有最优异的抗氧化能力;霸王花乙酸乙酯提取物(EAE)对伤寒杆菌有明显抑菌效果,其最小抑菌浓度为12.5mg/mL。结论:霸王花提取物具有作为天然抗氧化剂和天然抗菌素研究和开发的潜力。
Abstract: Objective:To study the antioxidant and antibacterial activities of different extracts from the flowers of Hylocereus undatus.Methods:The ethanol, acetone and ethyl acetate extracts were obtained by cold-extraction. The antioxidant activities were studied using DPPH, ABTS assay and reducing power assay was measured.; The antibacterial activities were studied using Kirby-Bauer and tube method.Results:Different extracts from the flowers of H. undatus exhibited antioxidant activities, and the most outstanding one was found to be the ethyl acetate extract ( EAE) ; the EAE extracts from the flowers of H.Undatus had the obvious bacteriostatic effect on the typhoid bacillus and the minimum inhibitory concentration was 12.5mg /mL.Conclusion:All the extracts could be employed as natural antioxidants and antimicrobial.
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参考文献(9)
[1] 国家中医药管理局中华本草编委会.中华本草[M].第六卷.上海:上海科学技术出版社, 2004:865. [2] 赵廉, 莫颖磊, 唯凌.霸王花的营养成分分析[J].食品研究与开发, 2001, 22 (1) :37. [3] 王宏磊.剑花皂苷提取纯化工艺和生物学功效研究[D].广州:华南师范大学, 2007. [4] 易衍, 巫鑫, 王英, 等.霸王花黄酮类成分研究[J].中草药2011, 34 (5) :712. [5] 胡春.黄酮类化合物的抗氧化性质[J].中国油脂, 1998, 21 (4) :18-20. [6] 李昌勤, 卢引, 尹震花, 等.6种南瓜栽培品种体外抗氧化活性研究[J].食品工业科技, 2012, 33 (15) :90-91. [7]Mazor D, Greenberg L, Shamir D, et al.Antioxidant properties of bucillamine:Possible mode of action[J].Biochem.Bioph.Res.Co., 2006, 349:1171-1172.
[8] 李萍, 祖丽皮亚·玉努斯.小黄杏色素抑菌作用的研究[J].食品工业科技, 2011, 32 (12) :139-140. [9] 郭群群, 杜桂彩, 李荣贵.紫苏叶挥发油杭菌活性研究[J].食品工业科技, 2003, 24 (9) :25-26.施引文献
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