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濒危赤水凤仙花叶绿体基因组特征及系统发育分析

花匠小妙招
2025-01-12 00:42

基金项目:

国家自然科学(32060364,32060366)；云南省重大科技专项(202102AE090052)；云南省园林植物遗传改良与高效繁育博士生导师团队资助

Characterization and Phylogenetic Comparative Analysis of the Complete Chloroplast Genome of Endangered Impatiens chishuiensis

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摘要:

【目的】研究赤水凤仙花的叶绿体基因组结构特征与系统发育位置，以期为赤水凤仙花植物种质资源保护、遗传多样性和系统发育研究提供科学依据。【方法】以中国特有种——赤水凤仙花为材料，基于赤水凤仙花叶绿体基因组序列，利用生物信息学软件，对叶绿体基因组进行组装、注释、基因特征、序列重复和系统发育分析。【结果】（1）赤水凤仙花叶绿体基因组呈典型的四分体结构，总GC含量37%，长152 892 bp；共编码113个基因，包括88个蛋白质编码基因、8个rRNA基因和17个tRNA基因。（2）共检测到76个SSR序列，以A、T组成为主；检测到50 842个密码子，其中以亮氨酸（Leu）最多，色氨酸（Tyr）最少。（3）凤仙花属分为2个亚属，为棒凤仙花亚属与凤仙花亚属，赤水凤仙花为棒凤仙花亚属且与太子凤仙花亲缘关系最近。【结论】赤水凤仙花为典型的四分体结构，SSR序列以A/T单碱基为主；系统发育分析结果将其归为棒凤仙花亚属。

Abstract:

【Objective】 To study the structural characteristics and phylogenetic position of the chloroplast genome of I. chishuiensis, with a view to providing scientific basis for the conservation of plant germplasm resources, genetic diversity and phylogenetic study of I. chishuiensis. 【Methods】 Based on the chloroplast genome sequence of I. chishuiensis flower, a Chinese endemic species, the chloroplast genome was assembled, annotated, gene characterised, sequence duplicated and phylogenetically analysed using bioinformatics software.【Results】 (1) The chloroplast genome of I. chishuiensis has a typical tetrameric structure with a total GC content of 37% and a length of 152 892 bp; it encodes a total of 113 genes, including 88 protein-coding genes, 8 rRNA genes, and 17 tRNA genes. (2) A total of 76 SSR sequences were detected in the sequence; 50 842 codons were detected, with leucine (Leu) being the most abundant and tryptophan (Tyr) being the least. (3) Impatiens was divided into two subgenera--subg. Clavicarpa and subg. Impatiens, and I. chishuiensis is a species of subg. Clavicarpa and has the closest affinity with I. alpicola. 【Conclusion】 I. chishuiensis is a typical tetrad structure, and the SSR sequence is dominated by the A/T single base; the results of phylogenetic analyses place it in the subg. Clavicarpa.

引用本文

兰文香,莫清,黄海泉.濒危赤水凤仙花叶绿体基因组特征及系统发育分析[J].西北植物学报,2024,44(12):1927-1936

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