首页 > 分享 > 超声波辅助提取山银花绿原酸工艺及其抗氧化性研究

超声波辅助提取山银花绿原酸工艺及其抗氧化性研究

花匠小妙招
2025-01-12 13:42

摘要: 以山银花为材料,采用高效液相法检测绿原酸,建立了超声波辅助提取绿原酸的工艺组合。通过单因素实验研究了提取时间、固液比、超声波功率、超声波频率四个主要因素对绿原酸提取的影响。在单因素实验的基础上采用L9(34)正交实验优选山银花绿原酸提取工艺条件。结果表明:室温下,溶剂为体积分数为50%的乙醇,超声波辅助山银花绿原酸的最佳提取工艺组合为超声波提取50min、固液比1∶50、超声波功率300W、超声波频率59kHz,在此条件下绿原酸提取率达到88.7624mg/g。验证实验表明该工艺组合提取率高,稳定性良好。山银花绿原酸提取物有一定的还原能力和较好的清除DPPH自由基的能力,是很好的功能性食品资源。

Abstract: The object of this research was to optimize ultrasonic- assisted extraction process of chlorogenic acid from Lonicera macranthoides Hand.-Mazz.The chlorogenic acid was tested by HPLC.To maximize the extraction of chlorogenic acid, main technological parameters like ultrasonic extraction time, ethanol concentration, solid /liquid ratio and temperature, were investigated by single factor method coupled with L 9 ( 34) orthogonal array design. Results showed that the optimal conditions of extracting chlorogenic acid from Lonicera macranthoides was 50% ethanol, ultrasonic power 300W, ultrasonic frequency 59kHz, extracting 50min, with a solid /liquid ratio of 1∶50.Under above conditions, the extraction yield of chlorogenic acid reached 88.7624 mg /g.It was proved that this extracting technology was reasonable and feasible.The results demonstrated that extract from Lonicera macranthoides had certain reduction capacity and potent DPPH radical scavenging capacity and could be used as antioxidants and in functional foods.

[1] 国家药典委员会.中华人民共和国药典 (一部) [S].北京:中国医药科技出版社, 2010:28. [2] 李荣, 周玉生, 匡双玉, 等.山银花提取物抗动脉粥样硬化成分研究[J].中国现代应用药学, 2011, 28 (2) :92-95. [3] 王林青, 张红英, 崔保安, 等.金银花、山银花绿原酸类提取物体外抗NDV作用研究[J].中国农学通报, 2011, 27 (19) :277-282. [4] 王林青, 崔保安, 张红英.金银花、山银花黄酮类提取物体外抗伪狂犬病病毒作用研究[J].中国畜牧兽医, 2011, 38 (3) :183-188. [5] 王志萍, 邓家刚, 王勤, 等.山银花研究的最新进展[J].广西中医学院学报, 2008, 11 (4) :59-61. [6]

Ronsted N, Strandgaard H, Jensen S R, et al.Chlorogenic acid from three species of Hydrostachys[J].Biochemical Systematics and Ecology, 2002, 30 (11) :1105-1108.

[7]

Award M A, Jager A, Westing L M.Flavonoid and chlorogenic acid levels in apple fruit:Characterisation of variation[J].Scientia Horticulturase, 2000, 83 (3-4) :249-263.

[8]

He C X, Cui H, Zhao G W.The determination of chlorogenic acid in cigarettes by inhibited chemiluminescence analysis[J].Analytica Chimica Acta, 1997, 351:241-246.

[9] 胡仲秋, 洪小迪, 岳田利.光皮木瓜绿原酸的提取及抗菌活性测定[J].食品科学, 2010, 31 (24) :8-13. [10]

Viviane M, Adriana F.Chlorogenic acids and related compounds in medicinal plants and infusions[J].Food Chemistry, 2009, 113:1370-1376.

[11]

Farah A, Donangelo C M.Phenolic compounds in coffee[J].Brazilian Journal of Plant Physiology, 2006, 18:23-36.

[12]

Wang G F, Shi L P, Ren Y D, et al.Anti-hepatitis B virus activity of chlorogenic acid, quinic acid and caffeic acid in vivo and in vitro[J].Antiviral Research, 2009, 83:186-190.

[13] 孙健, 吴国娟.绿原酸的研究进展[J].中兽医学杂志, 2009, 1:47-50. [14] 高锦明, 张鞍灵, 张康健, 等.绿原酸分布、提取与生物活性研究综述[J].西北林学院学报, 1999, 14 (2) :73-82. [15] 张元元.李进, 陈涛, 等.高效液相色谱法同时测定金银花中绿原酸和木犀草苷的含量[J].天津中医药大学学报, 2011, 30 (2) :107-109. [16] 康如龙, 刘倩, 苏小建, 等.冬瓜皮提取物抗氧化活性的研究[J].食品科技, 2013, 38 (3) :218-222. [17] 王小波, 何晓燕, 王梅, 等.火龙果皮总黄酮提取与体外抗氧化作用研究[J].食品科技, 2011, 32 (11) :156-163. [18] 朱兆友, 汝绍刚, 朱庆书.超声辅助提取藿香挥发油的研究[J].化学与生物工程, 2010, 27 (6) :85-87. [19] 肖卓炳, 郭瑞轲, 郭满满, 等.金银花中绿原酸超声微波双辅助提取工艺优化[J].食品科学, 2012, 33 (22) :111-114. [20] 刘玉萍.紫苏色素的超声波辅助提取工艺研究[J].食品工业, 2012, 33 (7) :19-21. [21] 周鸣谦, 王仁财.超临界CO2萃取柑橘落果中的辛弗林[J].中国食品学报, 2013, 13 (3) :78-83.