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建兰ABC基因家族鉴定及其在花发育过程中的表达模式分析

花匠小妙招
2024-11-08 23:28

摘要：

ABC基因家族编码一类定位于生物膜的转运蛋白，参与多种物质的转运，在植物生长发育和适应胁迫等生命活动中发挥重要作用。通过生物信息学方法对建兰ABC基因家族成员进行全基因鉴定，并基于转录组数据和实时荧光定量PCR技术（RT-qPCR）分析其在建兰花发育过程中的表达模式。结果表明，建兰基因组中共存在121个可分为8个亚族的ABC基因。所有的建兰ABC基因均具有至少1个保守的核苷酸结合结构域。建兰ABC基因家族成员不均匀分布于19条染色体上，2号和8号染色体上成员数目最多。串联重复事件是导致建兰ABC基因家族扩张的主要原因。建兰ABC基因启动子序列上存在多种环境和激素响应元件。CeABCB6、CeABCB30、CeABCG3、CeABCG54和CeABCI7基因的表达水平与建兰主要花香物质的释放量呈正相关。本研究为后续了解建兰ABC基因的功能奠定基础，并为建兰花香研究提供基因资源。

关键词: ABC转运蛋白, 建兰, 全基因组鉴定, 基因表达模式, 花发育, 花香

Abstract:

ATP-binding cassette（ABC）transporters are membrane transporter proteins that participate in the transportation of a huge range of substrates. Plant ABC genes function in many important biological processes of growth and development and abiotic stress resistance. We used bioinformatics methods to perform genome-wide identification of ABC gene family in Cymbidium ensifolium and analyze their expression patterns during flowering development based transcriptome data and RT-qPCR. In total，there were 121 ABC genes classified into 8 subfamilies in C. ensifolium genome. All ABC proteins had at least one conserved nucleotide binding domain（NBD）. The 121 ABC genes were unevenly distributed on 19 chromosomes，and the most ABC genes located on chromosome 2 and 8. Tandem duplication was found to be the main reason for the expansion of the ABC genes. Cis-acting element prediction revealed that the promoter sequences of ABC genes contained phytohormone and abiotic stress responsive elements. The expressions of CeABCB6，CeABCB30，CeABCG3，CeABCG54 and CeABCI7 were positively correlated with the volatilization of the key aromatic components in different flowering stages of the C. ensifolium. This study lays a foundation for further understanding the function of ABC genes and provides genetic resources for the study of C. ensifolium floral fragrance.

Key words: ABC transporters, Cymbidium ensifolium, genome-wide identification, gene expression pattern, flower development, floral fragrance