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真菌灰树花菌丝体转录组测序及分析.pdf

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2024-11-23 10:40

62017,Vol.38No.20
品字
※生物工程
真菌灰树花菌丝体转录组测序及分析
聂文强,吴天祥*,钟敏,芦红云
(1.贵州大学酿酒与食品工程学院,贵州贵阳550025:2.贵州大学明德学院,贵州贵阳550025)
摘要:灰树花多糖具有抗肿瘤、抗HV、抗氧化等作用,为探明灰树花多糖合成的遗传基础,采用 Illumina高通
量测序技术对灰树花转录组进行测序,获得74575910个 - reads,10.4G数据量;将测序数据进行 de novo拼接后得
到18077个 Unigene对所得 Unigene进行不同数据库比对,在Nr数据库中有11651个 Unigene被注释到,其中灰树
花转录组与变色栓菌相似序列最多(18.74%);有8332个 Unigene在GO数据库中注释到,分为细胞组分、分子功
能及生物学过程等3大类59个分支;与KEGG数据库比对,共有5200个 Unigene被注释,分属376条代谢通路。从
1807个 Jnigene中共找到155个简单重复序列( simple sequence repeat,SR)位点,其中单核苷酸重复最高,六
核苷酸重复最少,AT出现频率最高。本研究中在KEGG数据库注释到15个 Unigene与灰树花多糖合成有关,这些
Unigene及其注释信息为今后深入开展灰树花多糖代谢途径及相关功能基因等研究提供了依据。
关键词;灰树花;菌丝体;转录组测序;功能注释
Transcriptome Sequencing and Analysis of Grifola frondosa Mycelia
NE Wenqiang, WU Tianxianpl2,* ZHONG Min, LU Hongyun
(1. School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;
2. Mingde College of Guizhou University, Guiyang 550025, China)
Abstract: The exopolysaccharide of Grifola frondosa possesses anti-umor ant-hiv, and anti-oxidant effects. In order to
investigate the genetic basis for polysaccharide synthesis by G. frondosa, the transcriptome of G. frondosa was sequenced b
high-throughput sequencing technology A total of 74 575 910 reads and 10.4 G data were generated, which formed 18 077
Unigenes after de novo splicing. A total of 11 651 Unigenes were annotated in the Nr database. Among them, G. frondosa
transcriptome had the most similar sequence(18.%)to M tumefaciens A otal of 332 Unigenes were annotated in
the GO database, which were divided into 3 major categories molecular composition, molecular function and biological
processes, including 59 branches. Compared with the KEGG database, 5 200 Unigenes were annotated which belonged to
376 metabolic pathways. A total of 1 155 simple sequence repeat() loci were found in 18 077 Unigenes, among which
the highest repeat was single nucleotide whereas the repetion of hexanucleotide was the least, and the frequency of A/ was
the highest. In this study. 1 15 unigenes which have been annotated in the database of KEGG were related to the biosynthesis
of polysaccharide by G frondosa. These annotated Unigenes and the information about them can lay the foundation for
further studies on polysaccharide metabolism pathways and related functional genes in G. frondosa
Key words: Grifola frondosa; mycelium; transcriptome sequencing; functiona] annotation
DOI:10.7506spkx1002-6630-201720002
中图分类号:Q939.99
文献标志码;A
文章編号:1002-6630(2017)20-0006-06
引文格式:
聂文强,吴天样,钟敏,等.真薗灰树花菌丝体转录组测序及分析.食品科学,2017,38(20):6-11.DO:10.7506/
spkx1002-6630-201720002.http:/www.spkx.net.cn
NIE Wenqiang, WU Tianxiang, ZHONG Min, et al. Transcriptome sequencing and analysis of Grifola frondosa mycelia
FooScience,2017,38(20:6-1l.jnChinesewihEngishahbstact)DOI:10.7506pkx1002-6630-20172000.hy/wwpknetcn
原蛋白合成、提高机体免疫力四等生物活丝,“会
灰树花( Grifola frondosa)是一种营养丰富的药食两抗HIV]、抗氧化6、清除自由基回、降血糖、促进
用真菌。多糖是灰树花的主要活性成分,具有抗肿瘤、
收稿日期:2016-10-24
基金项目:国家自然科学基金地区科学基金项目(31460537)
作者简介:聂文强(1993-),男,硕士研究生,研究方向为生物信息学。 E-mail::648462055@qcom
*通信作者:吴天祥(1965一),男,教授,博士,研究方向为发酵工程和生物转化。E-mail:txwu@gzu.edu.cn
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