首页 > 分享 > 科学网—[转载]RNA干扰V1和C1提高番茄植株对番茄黄化曲叶病毒(TYLCV)的抗性

科学网—[转载]RNA干扰V1和C1提高番茄植株对番茄黄化曲叶病毒(TYLCV)的抗性

花匠小妙招
2024-11-23 11:33

RNA干扰V1和C1提高番茄植株对番茄黄化曲叶病毒(TYLCV)的抗性

李云洲 1 ,

岳宁波 1 ,

陈相儒 1 ,

李玉龙 4 ,

曹贺贺 3 ,

闫见敏 1, * ,

梁燕 2, *

作者信息

The Enhancement Resistance to TYLCV via RNA Interference V1 and C1 Gene of TYLCV in Tomato

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摘要

为了获得高抗番茄黄化曲叶病毒(TYLCV)番茄材料,本研究通过人工设计以TYLCV病毒基因V1、C1为靶标的双链RNA(dsRNA),通过农杆菌介导法转化番茄,探索RNAi技术在番茄抗TYLCV中的防御效果。结果表明,通过RNAi技术干扰TYLCVV1和C1基因可以延缓病毒症状的发生,提高番茄植株对TYLCV的抗性。田间试验结果发现,以TYLCV V1和C1为靶标的RNAi株系RNAi-CP-2和RNAi-Rep-12,不仅可以干扰病毒V1、C1的表达,而且可以干扰TYLCV其他4个基因V2、C2、C3和C4,表明RNAi技术可以在靶标基因附近传递,影响相邻其他基因的表达。本研究结果为番茄抗病毒研究奠定了理论基础。

Abstract

In order to obtain a highly resistant tomato material against Tomato yellow leaf curl virus (TYLCV), two double-stranded RNA (dsRNA) were designed to target the TYLCV virus genes V1 and C1, and were transformed in tomato by Agrobacterium-mediated, to explore the defense effect of RNAi technology in tomato against TYLCV. The results showed that interfering with V1 and C1 gene of TYLCV through RNAi can delay the occurrence of virus symptoms and improve the resistance of tomato plants to TYLCV. Field experiments found that RNAi strains, RNAi-CP-2 and RNAi-Rep-12, targeting TYLCVV1 and C1 gene, respectively, can not only interfere with the expression of V1 and C1, but also interfere with the other four genes of TYLCV, V2, C2, C3 and C4, which indicate that RNAi technology can ‘pass’ RNA interference along the target gene and affect the expression of other adjacent genes. This research lays a foundation for tomato antiviral research.

关键词

番茄黄化曲叶病毒 / 外壳蛋白(CP) / 复制相关蛋白(Rep) / RNAi / 抗病性

Key words

Tomato yellow leaf curl virus / coat protein / replication associated protein / RNA interference / disease resistance

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导出引用

李云洲 , 岳宁波 , 陈相儒 , 李玉龙 , 曹贺贺 , 闫见敏 , 梁燕. RNA干扰V1和C1提高番茄植株对番茄黄化曲叶病毒(TYLCV)的抗性[J]. 核农学报, 2021, 35(9): 2027-2034 https://doi.org/10.11869/j.issn.100-8551.2021.09.2027

LI Yunzhou , YUE Ningbo , CHEN Xiangru , LI Yulong , CAO Hehe , YAN Jianmin , LIANG Yan. The Enhancement Resistance to TYLCV via RNA Interference V1 and C1 Gene of TYLCV in Tomato[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2027-2034 https://doi.org/10.11869/j.issn.100-8551.2021.09.2027

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