茶树花萃取物降血脂及抗氧化性能研究
WANG Juan, YU Rui, HUANG Hui-hua. Reducing blood lipid and antioxidant activities of tea flower extracts[J]. Science and Technology of Food Industry, 2014, (05): 79-82. DOI: 10.13386/j.issn1002-0306.2014.05.019
Citation: WANG Juan, YU Rui, HUANG Hui-hua. Reducing blood lipid and antioxidant activities of tea flower extracts[J]. Science and Technology of Food Industry, 2014, (05): 79-82. DOI: 10.13386/j.issn1002-0306.2014.05.019
茶树花萃取物降血脂及抗氧化性能研究
详细信息
作者简介:王娟 (1981-) , 女, 博士, 讲师, 研究方向:食品科学与工程。;
中图分类号: R284
计量 文章访问数: 124 HTML全文浏览量: 18 PDF下载量: 529Reducing blood lipid and antioxidant activities of tea flower extracts
College of Light Industry and Food Sciences, South China University of Technology
摘要
摘要: 利用超临界CO2萃取得到茶树花浸膏,将超临界萃取后的茶树花渣用乙醇提取,得茶树花渣萃取物,研究二者的降血脂及抗氧化能力。以样品的体外结合胆酸盐能力衡量降血脂能力,以样品体外对超氧阴离子、羟基自由基与DPPH自由基的清除率衡量抗氧化能力。结果表明:茶树花浸膏及茶树花渣萃取物在本实验的最高浓度32mg/mL时对牛磺胆酸钠和甘氨胆酸钠的吸附率最大。茶树花渣乙醇萃取物对两种胆酸的吸附率与螯合胆酸型降血脂药物考来烯胺的吸附率更加接近。渣萃取物与考来烯胺对胆酸盐的吸附率均高于茶树花浸膏的胆酸盐吸附率。与抗氧化剂2,6-二叔丁基-4-甲基苯酚（BHT）相比,茶树花渣乙醇萃取物对超氧阴离子、羟基自由基的清除率更高,对DPPH自由基的清除率略低。茶树花渣萃取物与BHT对三种自由基的清除率均高于茶树花浸膏的清除率。
关键词: 茶树花 / 浸膏 / 茶树花渣 / 胆酸盐 / 抗氧化Abstract: Tea flower extract was prepared by supercritical CO2 extraction and the tea flower residue was extracted by ethanol.The reducing blood lipid and antioxidant activities of these two extracts were investigated. Bile salt-binding capacity in vitro and the clearance of superoxide anion, hydroxide radical and DPPH radical of the samples were tested in order to measure their reducing blood lipid and antioxidant activities.Results showed that tea flower extract and residue extract had the highest adsorption rate to sodium taurocholate and sodium glycocholate when the concentration was 32mg /mL ( the highest concentration in this experiment) . The residue extract had a similar adsorption rate to cholestyramine. The bile salt adsorption rate of residue extract and cholestyramine were higher than that of tea flower extract by supercritical CO2 extraction.Compared with BHT, the clearance rates of superoxide anion and hydroxide radical were higher in residue extract, but the clearance rates of DPPH radical was a little lower in residue extract.The three free radical clearances of the residue extract and BHT were higher than that of the tea flower extract by supercritical CO2 extraction.
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参考文献(6)
[1] 顾亚萍, 钱和.茶树花香气成分研究及其香精的制备[J].食品研究与开发, 2008, 29 (1) :187-190. [2] 余锐, 王娟, 黄惠华.响应曲面法优化微波辅助萃取制备茶树花浸膏的工艺研究[J].现代食品科技, 2011, 27 (11) :1375-1378转1315. [3] 余锐.茶树花的超临界CO2萃取及其浸膏的功能性研究[D].广州:华南理工大学, 2012. [4] 余锐, 王娟, 黄惠华.响应曲面法优化超临界CO2萃取茶树花工艺及萃取物成分分析[J].食品科学, 2012, 33 (12) :102-107. [5] 胡凯.茶叶功能性成分体外降血脂的机理研究[D].广州:华南理工大学, 2011. [6] 胡凯, 黄惠华.不同茶浸提液对胆酸盐的结合及其降血脂机理的研究[J].食品工业科技, 2011, 32 (8) :105-107, 111.施引文献
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