红花草莓‘莓红’花瓣花色苷积累及其MYB基因的表达分析
摘要：
以红花草莓品种‘莓红’为研究材料,观察花瓣发育过程中色泽及花色苷变化规律,同时对4个发育时期花瓣进行转录组测序,基于相关性筛选花色苷合成途径的关键功能基因及转录调控因子,结果表明：（1）花瓣发育过程中,花色苷总含量呈先升后降趋势,且在半开期达到最高;（2）花色苷合成途径关键功能基因FaPAL、Fa4CL-1、Fa4CL-2、FaDFR、Fa3GT表达水平与花色苷含量正相关;（3）R2R3-MYB基因FaMYB6和FaMYB90与花色苷含量呈正相关;（4）Pearson相关性分析表明FaMYB6与FaPAL、FaDFR显著正相关,FaMYB90与FaPAL、Fa4CL-1、Fa4CL-2、FaDFR、Fa3GT极显著正相关,FaMYB82则与上述5个结构基因之间均极显著负相关。综上,‘莓红’草莓花瓣发育过程中,花色苷积累引起花瓣颜色变红,FaMYB82、FaMYB6、FaMYB90可能通过调节FaPAL、Fa4CL、FaDFR和Fa3GT的表达来调控花瓣色泽形成。
关键词: 草莓, 红花, 花瓣, 花色苷, 生物合成, R2R3-MYB 转录因子
Abstract:
‘Meihong’which is a red-flowered strawberry variety was dynamically observed the anthocyanin contents during petals development. Moreover,we performed transcriptome sequencing on four development periods of petals for screening key functional genes and transcription factors in the anthocyanin synthesis pathway. The results showed that：(1) During the lifecycle of petal,the content of anthocyanin is sharply increased and reached the highest at the petal half-open period,and then decreased;(2) The key functional genes of the anthocyanin synthesis pathway,FaPAL,Fa4CL-1,Fa4CL-2,FaDFR,and Fa3GT,were positively correlated with the accumulation of anthocyanins in their expression patterns;(3) FaMYB6 and FaMYB90 were performed positively correlation with anthocyanin content;(4) Pearson correlation analysis showed that FaMYB6 was significantly positively correlated with FaPAL and FaDFR;FaMYB90 had a significant positive correlation with FaPAL,Fa4CL-1,Fa4CL-2,FaDFR and Fa3GT;FaMYB82 was significantly negatively correlated with FaPAL,Fa4CL-1,Fa4CL-2,FaDFR and Fa3GT. In summary,FaMYB82,FaMYB6,FaMYB90 regulate the transcriptional of anthocyanin synthesis genes facilitated the accumulation of anthocyanin caused the color formation of the petals in‘Meihong’strawberry.
Key words: strawberry, red-flower, petal, anthocyanin, biosynthesis, R2R3-MYB transcription factor
中图分类号:
S668.1
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