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海滨木槿种子无菌萌发及壮苗的培养基筛选

花匠小妙招
2024-12-15 03:30

摘要：

海滨木槿是东南沿海地区防风护堤林的首选树种,也是瘠薄山地水保林的良好树种。无菌壮苗的获取是建立高效再生体系的前提,可为植物资源保护和新品种选育提供基础。该研究以MS为基本培养基,比较柠檬酸、活性炭、三碘苯甲酸(TIBA)、6-苄氨基腺嘌呤(6-BA)、萘乙酸(NAA)、水解酪蛋白、维生素H、叶酸、噻苯隆(TDZ)和寡糖·链蛋白等添加物对海滨木槿种子萌发以及壮苗培养的影响。结果显示,种子萌发最佳配方为MS+6-BA 0.5 mg·L-1+ NAA 0.1 mg·L-1+水解酪蛋白0.1 g·L-1,萌发率从21.4%±7.8%提高到85.3%±9.1%;维生素、叶酸、柠檬酸的添加均未能促进种子的萌发。培养基MS+6-BA 0.1 mg·L-1+寡糖·链蛋白0.1 g·L-1+活性炭0.1%中的幼苗增殖系数最高,达7.0±2.6,且直立生长,茎枝较粗,叶片肥厚。值得注意的是,TDZ和NAA组合诱导出来的材料分化能力较强,可为后期海滨木槿诱导体胚成苗技术研究提供一定的参考。

关键词: 海滨木槿, 配方筛选, 6-BA, 水解酪蛋白, 寡糖·链蛋白

Abstract:

Hibiscus hamabo is not only a preferred species for windbreak forests in southeast coastal areas, but also a good species for water conservation forests in barren mountains. The acquisition of aseptic seedlings is a prerequisite for the establishment of efficient regeneration systems, which could provide a basis for plant resource conservation and new variety selection and breeding. In this study, MS was used as the basic medium to compare the effects of additives such as citric acid, activated carbon, triiodobenzoicacid (TIBA), 6-benzylaminopurine (6-BA), naphthalene acetic acid (NAA), casein hydrolyzed, vitamin H, folic acid, thidiazuron (TDZ), oligosaccharidic plant activator protein, etc. on the seed germination as well as the aseptic seedling cultivation of Hibiscus hamabo. The optimum formulation for seed germination was MS+6-BA (0.5 mg·L-1)+NAA (0.1 mg·L-1)+hydrolyzed casein (0.1 g·L-1), which increased the germination rate from 21.4%±7.8% to 85.3%±9.1%. Meanwhile, vitamin H, folic acid, and citric acid application failed to promote seed germination. Seedlings in medium of MS+6-BA (0.1 mg·L-1)+oligosaccharide chain protein (0.1 g·L-1)+activated carbon (0.1%) had the highest proliferation coefficient of 7.0±2.6 with thick stems, branches, and leaves. Notably, the combination of TDZ and NAA could induce the generation of Hibiscus hamabo with strong differentiation ability, which can provide a foundation for further developing the somatic embryo seedling technology of Hibiscus hamabo.

Key words: Hibiscus hamabo, formulation selection, 6-benzylaminopurine, hydrolyzed casein, oligosaccharide chain protein

中图分类号:

S682