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紫鸭跖草CpBURP的克隆、表达及生物信息学分析

花匠小妙招
2025-01-08 09:53

摘要：

BURP蛋白在植物生长发育、抵抗非生物胁迫中具有重要的作用。克隆CpBURP,并分析其在紫鸭跖草中的作用,为后期紫鸭跖草的BURP基因的深入研究奠定基础。根据紫鸭跖草转录组数据库进行筛选,并克隆CpBURP,通过对其进行生物信息学分析及表达分析。结果表明,1 383 bp的CpBURP编码含460个氨基酸的蛋白质,CpBURP蛋白属于亲水蛋白,含有一个BURP保守结构域和50个磷酸化位点,不含跨膜结构和信号肽区域。预测CpBURP蛋白作用于细胞质和过氧化物酶体。系统进化树显示,CpBURP蛋白与13种植物的BURP蛋白被分为6个亚类,CpBURP蛋白与甘蓝型油菜的BURP蛋白亲缘性最近。qRT-PCR结果显示,CpBURP在根中的表达量高于茎和叶,在Cu2+胁迫条件下,根中CpBURP的表达量明显高于对照组。CpBURP可能在紫鸭跖草的Cu2+胁迫应答反应中具有重要作用。

关键词: 紫鸭跖草, CpBURP, 基因克隆, 生物信息学, 表达分析

Abstract:

BURP protein plays an important role in the growth of plant and resistance to abiotic stress. Cloning a CpBURP and analyzing its role in Commelina purpurea may lay a foundation for further deeply studying the gene BURP in C. purpurea. Screening was performed based on the C. purpurea transcriptome database and a CpBURP was cloned,and then its bioinformatics and expressions were analyzed. The result showed the CpBURP of 1 383 bp encoded a protein with 460 amino acids. The hydrophilic CpBURP protein contained a BURP conserved domain and 50 phosphorylation sites,but no transmembrane structure and signal peptide region. It is predicted that CpBURP protein acted on the cytoplasm and peroxisomes. Phylogenetic tree analysis revealed that CpBURP protein and 13 plant BURP proteins were grouped into 6 subcategories,the CpBURP protein had the closest affinity to the BURP protein in Brassica napus. The results of qRT-PCR demonstrated that the expression of CpBURP in the root was higher than that in the stem and leaf. Under the Cu2+ stress,the expression of CpBURP in the roots was significantly higher than that in the control group. CpBURP may play an important role in the response of C. purpurea to Cu2+ stress.

Key words: Commelina purpurea, CpBURP, gene cloning, bioinformatics, expression analysis