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五味子木脂素超声提取工艺优化及其抗氧化和抗真菌的潜力

花匠小妙招
2025-02-24 03:45

摘要：

为探究五味子木脂素的超声提取工艺,及其抗氧化和抗真菌的能力,建立起一种基于液相色谱-质谱联用法(LC-MS/MS)同步检测五味子中5种木脂素含量的方法,在此基础上,基于4项单因素试验和中心复合设计-响应面法优化五味子木脂素的超声提取工艺,并评价其抗氧化和抗水产干制品中优势真菌的能力。结果表明:适宜超声提取五味子木脂素的工艺参数为乙醇体积分数70%,液料比12 mL·g-1,提取2次,每次超声10 min。在此条件下,五味子木脂素提取量为(9.45±0.11) mg·g-1。五味子木脂素提取液的总还原力,及对DPPH(1,1-二苯基-2-三硝基苯肼)、ABTS(2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸)自由基的清除能力分别是维生素C(VC)的2/5、1/2和1/3,对黄曲霉(Aspergillus flavus)、黑曲霉(Aspergillus niger)、橘青霉(Penicillium citrinum)、尖孢镰孢菌(Fusarium oxysporium)和厚垣镰孢菌(Fusarium chlamydosporum)的最低抑菌浓度(MIC)约为1.17 mg·mL-1,对哈茨木霉(Trichoderma harzianum)、扩展青霉(Penicillium expansum)和腐皮镰孢菌(Fusarium solani)的MIC约为2.34 mg·mL-1,说明其具有较强的抗氧化和抗水产干制品中优势真菌的能力。

关键词: 五味子木脂素, 液质联用, 超声提取, 响应面, 抗氧化

Abstract:

To explore the suitable ultrasonic extraction technology of Schisandra lignans and its inhibitory effect against oxidant and dominant fungi in dried aquatic products, a synchronous detection methods for 5 kinds of lignans was established via LC-MS/MS (liquid chromatography-tandem mass spectrometry). On the basis, four single-factor tests, and the central composite response surface test were adopted to optimize the ultrasonic extraction parameters of Schisandra lignans, and the anti-oxidant and anti-fungi activities of Schisandra lignans were analyzed. It was shown that the suitable extraction parameters included ethanol volume fraction of 70%, liquid to material ratio of 12 mL·g -1, extraction times of twice, and ultrasound time of 10 min per time. Under this condition, the extraction amount of lignans was (9.45±0.11) mg·g-1. The total reducing power, and DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS [2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)] free radical scavenging ability of Schisandra lignans were 2/5, 1/2 and 1/3 of vitamin C (VC), respectively. The minimum inhibitory concentration (MIC) of Schisandra lignans against Aspergillus flavus, Aspergillus niger, Penicillium citrinum, Fusarium oxysporium, Fusarium chlamydosporum was about 1.17 mg·mL -1, and the MIC of Schisandra lignans against Trichoderma harzianum, Penicillium expansum, Fusarium solani was about 2.34 mg·mL -1, which indicated that the extracted Schisandra lignans had strong inhibitory effect against oxidant and dominant fungi in dried aquatic products.

Key words: Schisandra lignans, liquid chromatography-tandem mass spectrometry, ultrasonic extraction, response surface, antioxidation

中图分类号:

TQ461

R917