Broccoli 'Fiolaro' (Brassica oleracea) an endangered typical Italian cultivar. A genetic analysis by SSR markers.
摘要
'Broccolo fiolaro' (Brassica oleracea L. convar. Italica) is a typical vegetable produced in a restricted hill country area around Creazzo (Vicenza) in north-eastern Italy. The cultivation of this vegetable dates back several centuries ago, but a very few farms are still involved in the production. 'Broccolo fiolaro' is a variety of broccoli highly valued for its agronomic and organoleptic features. Four 'Broccolo fiolaro' selections were characterized by simple sequence repeats (SSRs) molecular markers and compared with other five cultivated varieties of broccoli of the Italica group in order to reconstruct their genetic structure and evaluate the degree of similarity among and within populations. The analysis of 12 SSR polymorphic loci pointed out a low genetic variability among the four 'Broccolo fiolaro' selections. Moreover, the whole 'Fiolaro' group showed significant differentiation from the other broccoli cultivars and could be easily distinguished by cluster analysis. An assignment test on ~30 genotyped plants randomly chosen from each broccoli population correctly recognized the proper accession 88% of the time, proving this method to be useful for cultivar identification.展开▼
机译：'Broccolo fiolaro'（Brassica oleracea L. convar。Italica）是一种典型的蔬菜，产于意大利东北部Creazzo（维琴察）附近一个受限制的丘陵地区。这种蔬菜的种植可以追溯到几个世纪前，但是仍然有很少的农场参与生产。 'Broccolo fiolaro'是各种花椰菜，因其农艺学和感官特性而受到高度评价。通过简单的序列重复（SSR）分子标记表征了四个'Broccolo fiolaro'选择，并与Italica组的其他五个西兰花栽培品种进行了比较，以重建其遗传结构并评估种群之间和种群内的相似程度。对12个SSR多态性位点的分析指出，在四个“ Broccolo fiolaro”选择中，遗传变异性较低。此外，整个“ Fiolaro”组显示出与其他西兰花品种的显着差异，可以通过聚类分析轻松区分。对从每个西兰花种群中随机选择的约30种基因型植物进行的赋值测试可正确识别88％的正确种质，证明该方法可用于品种鉴定。展开▼
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